Table 4 FungiQuant quantitative validation results, obtained using pure plasmid standards and different mixed templates
From: FungiQuant: A broad-coverage fungal quantitative real-time PCR assay
Templates tested | Assay quantitative dynamic range | Average reaction efficiency (SD) | r2-value |
|---|---|---|---|
10 μl Reaction |  |  |  |
Plasmid standards-only | 25 – 107 copies | 91.80% (1.91%) | >0.99 |
Plasmid standards plus 0.5 ng human DNA | 25 – 107 copies | 93.20% (0.70%) | >0.99 |
Plasmid standards plus 1 ng human DNA | 25 – 107 copies | 97.02% (4.97%) | >0.99 |
Plasmid standards plus 5 ng human DNA | 25 – 107 copies | 92.85% (1.33%) | >0.99 |
Plasmid standards plus 10 ng human DNA | 25 – 107 copies | 91.21% (1.79%) | >0.99 |
C. albicans DNA-only | 10 fg – 10 ng | 94.75% (2.33%) | >0.98 |
C. albicans DNA plus 1 ng human DNA | 10 fg – 10 ng | 96.84% (1.93%) | >0.99 |
5 μl Reaction |  |  |  |
Plasmid standards-only | 25 – 107 copies | 92.17% (5.64%) | >0.98 |
Plasmid standards plus 1 ng human DNA | 25 – 107 copies | 94.21% (2.92%) | >0.99 |
Plasmid standards plus 10 ng human DNA | 50 – 108 copies | 92.64% (2.39%) | >0.99 |