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Table 4 FungiQuant quantitative validation results, obtained using pure plasmid standards and different mixed templates

From: FungiQuant: A broad-coverage fungal quantitative real-time PCR assay

Templates tested Assay quantitative dynamic range Average reaction efficiency (SD) r2-value
10 μl Reaction    
Plasmid standards-only 25 – 107 copies 91.80% (1.91%) >0.99
Plasmid standards plus 0.5 ng human DNA 25 – 107 copies 93.20% (0.70%) >0.99
Plasmid standards plus 1 ng human DNA 25 – 107 copies 97.02% (4.97%) >0.99
Plasmid standards plus 5 ng human DNA 25 – 107 copies 92.85% (1.33%) >0.99
Plasmid standards plus 10 ng human DNA 25 – 107 copies 91.21% (1.79%) >0.99
C. albicans DNA-only 10 fg – 10 ng 94.75% (2.33%) >0.98
C. albicans DNA plus 1 ng human DNA 10 fg – 10 ng 96.84% (1.93%) >0.99
5 μl Reaction    
Plasmid standards-only 25 – 107 copies 92.17% (5.64%) >0.98
Plasmid standards plus 1 ng human DNA 25 – 107 copies 94.21% (2.92%) >0.99
Plasmid standards plus 10 ng human DNA 50 – 108 copies 92.64% (2.39%) >0.99