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Table 2 Results from the in silico coverage analysis performed using two sequence matching conditions

From: FungiQuant: A broad-coverage fungal quantitative real-time PCR assay

 

Full length primer & probe (Stringent)

8-nt primer & full length probe (Relaxed)

Phylum

77.8%

88.9%

(7/9)

(8/9)

Sub-phylum

83.3%

94.4%

(15/18)

(17/18)

Class

92.3%

97.4%

(36/39)

(38/39)

Order

91.3%

96.9%

(116/127)

(123/127)

Family

91.9%

95.4%

(342/372)

(354/372)

Genus

91.4%

94.9%

(1018/1114)

(1057/1114)

Species

90.0%

94.2%

(2355/2617)

(2465/2617)