Figure 5From: Characterization of replication and conjugation of plasmid pWTY27 from a widely distributed Streptomyces speciesCharacterization of the binding reaction of TraA protein with clt DNA by EMSA and footprinting. (a). Characteristics of a clt sequence on pWTY27 for plasmid transfer. Possible DC (direct repeat) and IC (inverted repeat) sequences are shown. (b) as Figure2(b). (c) as Figure2(c). The amounts of TraA protein used in lanes 1–5 were 0, 0.6, 1.4, 2.8 and 4.2 μg, respectively. Two sequences protected by TraA from digestion with DNaseI are shown.Back to article page