Figure 1

Comparison of Minimum spanning tree constructed either from 7 MLST markers (housekeeping genes) or from 17 MLVA markers, for 331 S. pneumoniae isolates. A: The minimum spanning tree was constructed with a categorical coefficient. Each coloured circle represents a different MLVA type (MT). The width of the line reflects the genetic distance between ST (heavy short lines connect SLVs, thin longer lines connect DLVs, and dotted lines indicate the most likely connection between 2 STs differing by more than 2 loci). B: The minimum spanning tree was constructed with a categorical coefficient. Each circle represents a different MLST type (ST). The colour of a circle and the line clustering the MT with the same colour are corresponding to identical sequence type (ST). Same colours design STs in Figure 1A. Size of the circle reflects the number of isolates designed in italic numbers within parenthesis, while the width of the line reflects the genetic distance between MT (heavy short lines connect SLVs, thin longer lines connect DLVs, and dotted lines indicate the most likely connection between 2 STs differing by more than 2 loci). The number of loci that differ between two MTs is indicated on the lines connecting the MTs. Clonal complexes (CC) were defined as MTs having a maximum distance of changes at 2 loci and a minimum cluster size of 2 types. Each CC as a cluster is shaded in a different colour.