Antiadhesive activity of the biosurfactant pseudofactin II secreted by the Arctic bacterium Pseudomonas fluorescens BD5

Table 2 Microbial adhesion inhibition in the microtiter plate by purified pseudofactin II.

Microorganism	Microbial adhesion inhibition (%)
	Pseudofactin II concentration (mg/ml)							Control (PBS)
	0.500	0.35	0.250	0.200	0.150	0.075	0.035	0
Escherichia coli ATCC 25922	66 ± 0.13	65 ± 0.13	65 ± 0.07	64 ± 0.07	62 ± 0.07	58 ± 0.07	55 ± 0.20	0
Escherichia coli ATCC 10536	80 ± 0.13	80 ± 0.13	80 ± 0.13	77 ± 0.13	72 ± 0.13	65 ± 0.07	39 ± 0.13	0
Escherichia coli 17-2	72 ± 0.33	71 ± 0.13	71 ± 0.13	69 ± 0.13	68 ± 0.07	64 ± 0.07	64 ± 0.13	0
Enterococcus faecalis ATCC 29212	70 ± 0.20	68 ± 0.20	68 ± 0.13	57 ± 0.13	55 ± 0.07	54 ± 0.07	42 ± 0.13	0
Enterococcus faecalis JA/3	36 ± 0.13	36 ± 0.13	34 ± 0.13	31 ± 0.13	22 ± 0.13	18 ± 0.20	15 ± 0.07	0
Enterococcus hirae ATCC 10541	71 ± 0.07	71 ± 0.20	71 ± 0.20	67 ± 0.20	66 ± 0.13	61 ± 0.07	58 ± 0.13	0
Staphylococcus epidermidis KCTC 1917	55 ± 0.13	45 ± 0.07	45 ± 0.07	33 ± 0.13	32 ± 0.07	31 ± 0.13	29 ± 0.13	0
Proteus mirabilis ATCC 21100	90 ± 0.20	90 ± 0.33	90 ± 0.33	89 ± 0.13	87 ± 0.07	85 ± 0.20	84 ± 0.20	0
Candida albicans ATCC 20231	92 ± 0.07	89 ± 0.07	81 ± 0.07	71 ± 0.13	68 ± 0.07	47 ± 0.20	45 ± 0.20	0
Candida albicans SC5314	99 ± 0.07	98 ± 0.07	98 ± 0.07	97 ± 0.07	96 ± 0.07	88 ± 0.07	87 ± 0.07	0

PBS was used as control and set at 0% as no microbial inhibition occurs. Values ± confidence interval, n = 9