From: FK506 biosynthesis is regulated by two positive regulatory elements in Streptomyces tsukubaensis
Strain | Plasmid | Promoter | Gene | # if isolates | Description |
---|---|---|---|---|---|
WT | 11 | Control | |||
WT pSET152 | pSET152 | 22 | Control | ||
WT allN | pDG1 | P ermE* | allN | 28 | Over-expression |
WT allN+mgl | pDG2 | P ermE* | allN+mgl | 25 | Over-expression |
WT fkbR | pDG3 | P ermE* | fkbR | 24 | Over-expression |
WT fkbN | pDG4 | P ermE* | fkbN | 29 | Over-expression |
ΔallN | pDG5 | ΔallN | 7 | Gene inactivation | |
ΔallNΔfkbN | pDG5, 8 | ΔallN ΔfkbN | 2 | Gene inactivation | |
ΔfkbN | pDG8 | ΔfkbN | 10 | Gene inactivation | |
ΔfkbR | pDG6 | ΔfkbR | 6 | Gene inactivation | |
ΔfkbRΔfkbN | pDG6, 8 | ΔfkbR ΔfkbN | 2 | Gene inactivation | |
ΔfkbR | pDG3 | P ermE* | fkbR | 18 | Complementation |
ΔfkbN | pDG4 | P ermE* | fkbN | 33 | Complementation |
ΔfkbRΔfkbN | pDG4 | P ermE* | fkbN | 9 | Complementation |
WT | pSET152 | rppA | 12 | Negative control | |
ΔfkbR | pMB1 | P ermE* | rppA | 9 | Positive control |
pMB2 | P allA | rppA | 15 | Promoter activity | |
pMB3 | P fkbR | rppA | 15 | Promoter activity | |
ΔfkbN | pMB4 | P fkbN | rppA | 12 | Promoter activity |
pMB5 | P fkbB | rppA | 15 | Promoter activity | |
pMB6 | P fkbG | rppA | 15 | Promoter activity |