PCR-DGGE analysis with Lactobacillus-specific primers. Analysis was conducted on the vaginal samples collected at 33rd (W33) and 37th (W37) week of gestation from 15 women supplemented with the probiotic VSL#3 [(P) N. 1–15] and 12 control women [(C) N. 16–27]. N: woman number; W: week of gestation; T: type of supplementation. (A) PCR-DGGE fingerprints. M, external reference marker. Band L16 corresponds to L. helveticus (GenBank accession number: AB571603) (B) Dendrogram of the DGGE profiles shown in panel A. Pearson correlation was used to calculate the similarity in DGGE profiles.