| |
Strains
|
|---|
| |
UCD 67-385
|
385-cyp61(+/−)
|
385-cyp61(−/−)
|
|---|
|
Cultivation time (h)
|
24
|
72
|
120
|
24
|
72
|
120
|
24
|
72
|
120
|
|
Ergosterol*
|
4.74±0.53
|
3.10±0.09
|
2.24±0.42
|
3.19±0.48
|
2.87±0.32
|
2.91±0.34
|
ND
|
ND
|
ND
|
|
Peak 2**
|
0.23±0.03
|
0.030±0.003
|
0.10±0.05
|
0.62±0.05
|
0.11±0.03
|
0.12±0.02
|
6.34±2.68
|
2.36±0.74
|
2.39±0.27
|
|
Peak 3***
|
0.19±0.04
|
ND
|
0.09±0.02
|
0.11±0.01
|
0.02±0.01
|
0.01±0.003
|
1.65±0.84
|
1.91±0.51
|
2.20±0.42
|
|
Total Sterols
|
5.16±0.57
|
3.13±0.09
|
2.40±0.49
|
3.96±0.44
|
2.99±0.35
|
3.04±0.36
|
8.14±3.42
|
4.27±1.24
|
4.59±0.70
|
| |
Strains
| | | |
| |
CBS 6938
|
CBS
-
cyp61
(−)
| | | |
|
Cultivation time (h)
|
24
|
72
|
120
|
24
|
72
|
120
| | | |
|
Ergosterol*
|
3.31±0.60
|
2.39±0.56
|
2.37±0.11
|
ND
|
ND
|
ND
| | | |
|
Peak 2**
|
0.07±0.04
|
0.06±0.02
|
0.06±0.01
|
2.00±0.34
|
1.24±0.02
|
1.23±0.04
| | | |
|
Peak 3***
|
0.03±0.001
|
0.02±0.01
|
0.03±0.01
|
2.38±0.29
|
2.60±0.08
|
3.05±0.17
| | | |
|
Total Sterols
|
3.45±0.56
|
2.41±0.59
|
2.46±0.11
|
4.38±0.61
|
3.85±0.1
|
4.28±0.21
| | | |
| |
Strains
| | | |
| |
AVHN2
|
AV2
-
cyp61
(−)
| | | |
|
Cultivation time (h)
|
24
|
72
|
120
|
24
|
72
|
120
| | | |
|
Ergosterol*
|
1.59±0.62
|
2.35±0.59
|
3.27±0.38
|
ND
|
ND
|
ND
| | | |
|
Peak 2**
|
ND
|
0.04±0.01
|
0.04±0.01
|
1.68±0.78
|
2.10±0.32
|
1.78±0.13
| | | |
|
Peak 3***
|
ND
|
ND
|
ND
|
1.39±0.82
|
2.27±0.18
|
2.39±0.52
| | | |
|
Total Sterols
|
1.59±0.62
|
2.39±0.59
|
3.31±0.39
|
3.16±1.70
|
4.36±0.49
|
4.11±0.64
| | | |
- Table shows the mean values ± standard deviations of three independent experiments. Retention time: *: 18 min; **: 15 min; ***: 22 min. ND: Not detected.
