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Table 3 Sterol composition according to their RP-HPLC profile of wild-type and cyp61 X . dendrorhous mutant strains (in mg/g dry yeast weight)

From: Enhancement of carotenoid production by disrupting the C22-sterol desaturase gene (CYP61) in Xanthophyllomyces dendrorhous

  Strains
  UCD 67-385 385-cyp61(+/−) 385-cyp61(−/−)
Cultivation time (h) 24 72 120 24 72 120 24 72 120
Ergosterol* 4.74±0.53 3.10±0.09 2.24±0.42 3.19±0.48 2.87±0.32 2.91±0.34 ND ND ND
Peak 2** 0.23±0.03 0.030±0.003 0.10±0.05 0.62±0.05 0.11±0.03 0.12±0.02 6.34±2.68 2.36±0.74 2.39±0.27
Peak 3*** 0.19±0.04 ND 0.09±0.02 0.11±0.01 0.02±0.01 0.01±0.003 1.65±0.84 1.91±0.51 2.20±0.42
Total Sterols 5.16±0.57 3.13±0.09 2.40±0.49 3.96±0.44 2.99±0.35 3.04±0.36 8.14±3.42 4.27±1.24 4.59±0.70
  Strains    
  CBS 6938 CBS - cyp61 (−)    
Cultivation time (h) 24 72 120 24 72 120    
Ergosterol* 3.31±0.60 2.39±0.56 2.37±0.11 ND ND ND    
Peak 2** 0.07±0.04 0.06±0.02 0.06±0.01 2.00±0.34 1.24±0.02 1.23±0.04    
Peak 3*** 0.03±0.001 0.02±0.01 0.03±0.01 2.38±0.29 2.60±0.08 3.05±0.17    
Total Sterols 3.45±0.56 2.41±0.59 2.46±0.11 4.38±0.61 3.85±0.1 4.28±0.21    
  Strains    
  AVHN2 AV2 - cyp61 (−)    
Cultivation time (h) 24 72 120 24 72 120    
Ergosterol* 1.59±0.62 2.35±0.59 3.27±0.38 ND ND ND    
Peak 2** ND 0.04±0.01 0.04±0.01 1.68±0.78 2.10±0.32 1.78±0.13    
Peak 3*** ND ND ND 1.39±0.82 2.27±0.18 2.39±0.52    
Total Sterols 1.59±0.62 2.39±0.59 3.31±0.39 3.16±1.70 4.36±0.49 4.11±0.64    
  1. Table shows the mean values ± standard deviations of three independent experiments. Retention time: *: 18 min; **: 15 min; ***: 22 min. ND: Not detected.