Skip to main content

Table 3 Sterol composition according to their RP-HPLC profile of wild-type and cyp61 X . dendrorhous mutant strains (in mg/g dry yeast weight)

From: Enhancement of carotenoid production by disrupting the C22-sterol desaturase gene (CYP61) in Xanthophyllomyces dendrorhous

 

Strains

 

UCD 67-385

385-cyp61(+/−)

385-cyp61(−/−)

Cultivation time (h)

24

72

120

24

72

120

24

72

120

Ergosterol*

4.74±0.53

3.10±0.09

2.24±0.42

3.19±0.48

2.87±0.32

2.91±0.34

ND

ND

ND

Peak 2**

0.23±0.03

0.030±0.003

0.10±0.05

0.62±0.05

0.11±0.03

0.12±0.02

6.34±2.68

2.36±0.74

2.39±0.27

Peak 3***

0.19±0.04

ND

0.09±0.02

0.11±0.01

0.02±0.01

0.01±0.003

1.65±0.84

1.91±0.51

2.20±0.42

Total Sterols

5.16±0.57

3.13±0.09

2.40±0.49

3.96±0.44

2.99±0.35

3.04±0.36

8.14±3.42

4.27±1.24

4.59±0.70

 

Strains

   
 

CBS 6938

CBS - cyp61 (−)

   

Cultivation time (h)

24

72

120

24

72

120

   

Ergosterol*

3.31±0.60

2.39±0.56

2.37±0.11

ND

ND

ND

   

Peak 2**

0.07±0.04

0.06±0.02

0.06±0.01

2.00±0.34

1.24±0.02

1.23±0.04

   

Peak 3***

0.03±0.001

0.02±0.01

0.03±0.01

2.38±0.29

2.60±0.08

3.05±0.17

   

Total Sterols

3.45±0.56

2.41±0.59

2.46±0.11

4.38±0.61

3.85±0.1

4.28±0.21

   
 

Strains

   
 

AVHN2

AV2 - cyp61 (−)

   

Cultivation time (h)

24

72

120

24

72

120

   

Ergosterol*

1.59±0.62

2.35±0.59

3.27±0.38

ND

ND

ND

   

Peak 2**

ND

0.04±0.01

0.04±0.01

1.68±0.78

2.10±0.32

1.78±0.13

   

Peak 3***

ND

ND

ND

1.39±0.82

2.27±0.18

2.39±0.52

   

Total Sterols

1.59±0.62

2.39±0.59

3.31±0.39

3.16±1.70

4.36±0.49

4.11±0.64

   
  1. Table shows the mean values ± standard deviations of three independent experiments. Retention time: *: 18 min; **: 15 min; ***: 22 min. ND: Not detected.