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Table 2 Strains and Plasmids used and built in this work

From: Enhancement of carotenoid production by disrupting the C22-sterol desaturase gene (CYP61) in Xanthophyllomyces dendrorhous

  Genotype or relevant features Source or reference
Strains:   
E. coli:   
DH-5α F- φ80d lacZΔM15Δ (lacZY-argF) U169 deoR recA1 endA1 hsdR17(rk- mk+) phoA supE44l- thi-1 gyrA96 relA1 [52]
X. dendrorhous:   
UCD 67-385 ATCC 24230, wild type. ATCC
Diploid strain [30]
385-cyp61(+/−) (385-CYP61/cyp61hph). Heterozygote transformant derived from UCD 67–385 containing an allele of the CYP61 locus interrupted with a hygromycin B resistance cassette. This work
385-cyp61(−/−) (385-cyp61hph/cyp61zeo). Homozygote transformant derived by transformation of 385-cyp61+/− with both CYP61 alleles interrupted, one with a hygromycin B resistance cassette and the other with a zeocin resistance cassette. This work
CBS 6938 ATCC 96594, wild type. ATCC
CBS-cyp61(−) (CBS-cyp61hph). Hemizygote transformant derived from CBS 6938. The single CYP61 locus was interrupted with a hygromycin B resistance cassette. This work
AVHN2* Chilean native isolate, wild type. Our Lab collection
Av2-cyp61(−) (Av2-cyp61zeo). Hemizygote transformant derived from AVHN2. The single CYP61 locus was interrupted with a zeocin resistance cassette. This work
UCD 67-210 ATCC 24202, wild type (Phaffia rhodozyma) ATCC
VKM Y-2786 Wild-type strain. VKM
ANCH03* Chilean Antarctic native isolate, wild type. Our Lab collection
ANCH07* Chilean Antarctic native isolate, wild type. Our Lab collection
ANCH10* Chilean Antarctic native isolate, wild type. Our Lab collection
Plasmids:   
pBluescript SK- (pBS) ColE1 ori; AmpR; cloning vector with blue-white selection Stratagene
pMN-hph pBS containing at the EcoRV site a cassette of 1.8 kb bearing the E. coli-Hygromycin B resistance (hph) gene under EF-1 α promoter and GPD transcription terminator of X. dendrorhous. [31]
pIR-zeo pBS containing at the EcoRV site a cassette of 1.2 kb bearing the Streptoalloteichus hindustanus Zeocin resistance Sh ble gene under EF-1 α promoter and GPD transcription terminator of X. dendrorhous. This work
pBS-gCyp61 pBS containing at the EcoRV site a 4,224 bp DNA fragment containing the X. dendrorhous CYP61 gene amplified by PCR with primers CYP61up2.F and CYP61dw2.R. This work
pBS-cyp61/Hyg pBS-gCyp61 bearing the Hygromycin B resistance cassette at the EcoRV site that interrupts the CYP61 gene. This work
pBS-cyp61/Zeo pBS-gCyp61 bearing the Zeocin resistance cassette at the EcoRV site that interrupts the CYP61 gene. This work
pBS-cCyp61 pBS bearing the cDNA of the CYP61 gene. The cDNA measures 1,752 bp with an ORF of 1,581 bp. This work
  1. *: X. dendrorhous Chilean native isolates confirmed by ITS, D1/D2 and IGS regions sequences. The following abbreviations are used for microorganism culture collections: CBS, Centraalbureau voor Schimmelcultures, Utrecht, Netherlands; ATCC, American Type Culture Collection, Manassas, USA; UCD, Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California at Davis, Davis, USA; VKM, The All-Russian Collection of Microorganisms, Moscow, Russia.