Table 2 Strains and Plasmids used and built in this work

Strains:

E. coli:

DH-5α

F- φ80d lacZΔM15Δ (lacZY-argF) U169 deoR recA1 endA1 hsdR17(rk- mk+) phoA supE44l- thi-1 gyrA96 relA1

[52]

X. dendrorhous:

UCD 67-385

ATCC 24230, wild type.

ATCC

Diploid strain [30]

385-cyp61^(+/−)

(385-CYP61/cyp61^hph). Heterozygote transformant derived from UCD 67–385 containing an allele of the CYP61 locus interrupted with a hygromycin B resistance cassette.

This work

385-cyp61^(−/−)

(385-cyp61^hph/cyp61^zeo). Homozygote transformant derived by transformation of 385-cyp61^+/− with both CYP61 alleles interrupted, one with a hygromycin B resistance cassette and the other with a zeocin resistance cassette.

This work

CBS 6938

ATCC 96594, wild type.

ATCC

CBS-cyp61⁽⁻⁾

(CBS-cyp61^hph). Hemizygote transformant derived from CBS 6938. The single CYP61 locus was interrupted with a hygromycin B resistance cassette.

This work

AVHN2^*

Chilean native isolate, wild type.

Our Lab collection

Av2-cyp61⁽⁻⁾

(Av2-cyp61^zeo). Hemizygote transformant derived from AVHN2. The single CYP61 locus was interrupted with a zeocin resistance cassette.

This work

UCD 67-210

ATCC 24202, wild type (Phaffia rhodozyma)

ATCC

VKM Y-2786

Wild-type strain.

VKM

ANCH03^*

Chilean Antarctic native isolate, wild type.

Our Lab collection

ANCH07^*

Chilean Antarctic native isolate, wild type.

Our Lab collection

ANCH10^*

Chilean Antarctic native isolate, wild type.

Our Lab collection

Plasmids:

pBluescript SK- (pBS)

ColE1 ori; AmpR; cloning vector with blue-white selection

Stratagene

pMN-hph

pBS containing at the EcoRV site a cassette of 1.8 kb bearing the E. coli-Hygromycin B resistance (hph) gene under EF-1 α promoter and GPD transcription terminator of X. dendrorhous.

[31]

pIR-zeo

pBS containing at the EcoRV site a cassette of 1.2 kb bearing the Streptoalloteichus hindustanus Zeocin resistance Sh ble gene under EF-1 α promoter and GPD transcription terminator of X. dendrorhous.

This work

pBS-gCyp61

This work

pBS-cyp61/Hyg

pBS-gCyp61 bearing the Hygromycin B resistance cassette at the EcoRV site that interrupts the CYP61 gene.

This work

pBS-cyp61/Zeo

pBS-gCyp61 bearing the Zeocin resistance cassette at the EcoRV site that interrupts the CYP61 gene.

This work

pBS-cCyp61

pBS bearing the cDNA of the CYP61 gene. The cDNA measures 1,752 bp with an ORF of 1,581 bp.

This work