Table 2 Primers used in this study
Primer | Sequence (5′-3′) |
|---|---|
JAF 401 | #GAG GAA TAA TAA ATG CTG ATT CTG ACT CGT CGA GTT GGT GAG |
JAF 402 | *TTA ATG ATG ATG ATG ATG ATG GTA ACT GGA CTG CTG GGA TTT |
JAF 403 | #GAG GAA TAA TAA TTA ATA TTA TCA AGA AAA GAA A |
JAF 404 | *TTA ATG ATG ATG ATG ATG ATG TTT GAT TAG TTT TTT GCT TA |
#Underlined nucleotides indicate a manufacturer recommended addition to remove vector encoded N-terminal leader sequence for expression of the native protein. | |
*Underlined nucleotides indicate a manufacturer recommended addition to remove vector encoded C-terminal V5 epitope and add a polyhistidine tag for expression of the native protein. |