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Table 2 Primers used in this study

From: Campylobacter jejuni CsrA complements an Escherichia coli csrAmutation for the regulation of biofilm formation, motility and cellular morphology but not glycogen accumulation

Primer

Sequence (5′-3′)

JAF 401

#GAG GAA TAA TAA ATG CTG ATT CTG ACT CGT CGA GTT GGT GAG

JAF 402

*TTA ATG ATG ATG ATG ATG ATG GTA ACT GGA CTG CTG GGA TTT

JAF 403

#GAG GAA TAA TAA TTA ATA TTA TCA AGA AAA GAA A

JAF 404

*TTA ATG ATG ATG ATG ATG ATG TTT GAT TAG TTT TTT GCT TA

 

#Underlined nucleotides indicate a manufacturer recommended addition to remove vector encoded N-terminal leader sequence for expression of the native protein.

 

*Underlined nucleotides indicate a manufacturer recommended addition to remove vector encoded C-terminal V5 epitope and add a polyhistidine tag for expression of the native protein.