Figure 4From: Campylobacter jejuni CsrA complements an Escherichia coli csrAmutation for the regulation of biofilm formation, motility and cellular morphology but not glycogen accumulationCsrACJrestores wild type E. coli biofilm formation. Biofilm formation in MG1655[pBAD], TRMG1655[pBAD], TRMG1655[pBADcsrAEC], and TRMG1655[pBADcsrACJ] were assessed in either polystyrene culture tubes (top panel; both side and bottom view of polystyrene culture tubes are represented.) or 96-well polystyrene microtiter dishes (quantitated in graph) using crystal violet staining after static growth for 48 hours at 26°C. Bottom Panel) Expression of his-tagged CsrAEC and CsrACJ in TRMG1655 was confirmed by western blot using anti-his primary antibodies. Presence (+) or absence (−) of inducible CsrAEC or CsrACJ in each strain is shown beneath the panels. ANOVA was performed to determine statistical significance of TRMG1655 expressing recombinant CsrAEC or CsrACJ versus TRMG1655[pBAD] (* p<0.001).Back to article page