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Figure 4 | BMC Microbiology

Figure 4

From: Campylobacter jejuni CsrA complements an Escherichia coli csrAmutation for the regulation of biofilm formation, motility and cellular morphology but not glycogen accumulation

Figure 4

CsrACJrestores wild type E. coli biofilm formation. Biofilm formation in MG1655[pBAD], TRMG1655[pBAD], TRMG1655[pBADcsrAEC], and TRMG1655[pBADcsrACJ] were assessed in either polystyrene culture tubes (top panel; both side and bottom view of polystyrene culture tubes are represented.) or 96-well polystyrene microtiter dishes (quantitated in graph) using crystal violet staining after static growth for 48 hours at 26°C. Bottom Panel) Expression of his-tagged CsrAEC and CsrACJ in TRMG1655 was confirmed by western blot using anti-his primary antibodies. Presence (+) or absence (−) of inducible CsrAEC or CsrACJ in each strain is shown beneath the panels. ANOVA was performed to determine statistical significance of TRMG1655 expressing recombinant CsrAEC or CsrACJ versus TRMG1655[pBAD] (* p<0.001).

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