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Figure 3 | BMC Microbiology

Figure 3

From: Campylobacter jejuni CsrA complements an Escherichia coli csrAmutation for the regulation of biofilm formation, motility and cellular morphology but not glycogen accumulation

Figure 3

CsrACJcomplements the motility defect of the E. coli csrA mutant. The motility of MG1655[pBAD], TRMG1655[pBAD], TRMG1655[pBADcsrAEC], and TRMG1655[pBADcsrACJ] was assessed on semisolid (0.35%) LB agar after 14 hours of growth at 30°C. Top Panel) Representative motility zones are shown, along with a graph of the measured zones of motility in three separate repetitions (n = 20/ repetition). Bottom Panel) Expression of his-tagged CsrAEC and CsrACJ in TRMG1655 was confirmed by western blot using anti-his primary antibodies. Presence (+) or absence (−) of inducible CsrAEC or CsrACJ in each strain is shown beneath the panels. ANOVA was performed to determine statistical significance of TRMG1655 expressing recombinant CsrAEC or CsrACJ versus TRMG1655[pBAD] (** p<0.0001).

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BMC Microbiology

ISSN: 1471-2180

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