Figure 2From: Campylobacter jejuni CsrA complements an Escherichia coli csrAmutation for the regulation of biofilm formation, motility and cellular morphology but not glycogen accumulationGlycogen accumulation in wild type, csrA mutant, and complemented mutant strains of E. coli. Top Panel) MG1655[pBAD], TRMG1655[pBAD], TRMG1655[pBADcsrAEC], and TRMG1655[pBADcsrACJ] were spotted onto Kornberg agar supplemented with 2% glycerol and 0.002% L-arabinose and incubated at 37°C overnight. The following day, the strains were stained for glycogen accumulation by inverting over iodine crystals. Bottom Panel) Expression of his-tagged CsrAEC and CsrACJ in TRMG1655 was confirmed by western blot using anti-his primary antibodies. Presence (+) or absence (−) of inducible CsrAEC or CsrACJ in each strain is shown beneath the panels.Back to article page