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Table 1 Primer list

From: Genotyping of B. licheniformisbased on a novel multi-locus sequence typing (MLST) scheme

Primer Sequence (5’-3’) Amplicon size (bp) Target gene (s) Target (from-to bp)in NC_00627
adk1L GGT AAA GGG ACA CAG GCT GA 518 BL01030: adk ;adenylate kinase 144018-144535
adk1R TCG AGT AAA GGC TGG GTT TG
ccpA2L TAT GAT GTA GCA CGC GAA GC 604 BL00444: ccpA; transcriptional regulator (compl) 3007794-3008397
ccpA4R TAT CCC CAA GCG CTC TTT TA
recF2L ACG GTT CTG TTC CCA TTC AG 603 BL00079: recF; recombination protein F 3791-4393
recF2R CAT CAC GGC CAT TGA CAT AG
sucC2L GGG TCC CGA CGG CCA ACA AA 595 BL01285: sucC; succinyl-CoA synthetase, subunit beta 1786001-1786595
sucC2R GGC CGG TTC CCC TCC GTA GT
rpoB-f AGG TCA ACT AGT TCA GTA TGG ACG[1] 580 BL02798: rpoB; DNA-directed RNA polymerase, subunit beta 120955-121534
rpoB-r AAG AAC CGT AAC CGG CAA CTT[1]
spo0A1L GAA GTG CTT GGT GTC GCA TA 627 BL01518: spo0A; response regulator 2512117-2512743
spo0A1R TGT GTA GCC GAA AAG TGA CG
pyrE2L AAA TCA AAG CGG TTT TCC TG 575 BL02280: pyrE; orotate phosphoribosyltransferase 1734961-1735535
pyrE1R AGG ATC CGC TTT CCA TTC TT
glpT1L CTT ACG GGC TGA GCA AGT TC 593 BL00185: glpT; glycerol-3-phosphate permease 4094701-4095293
glpT1R CAC GAA AAT GTT GGC AAG TG
3gyrBF ATC GTT GAG GGT GAC TCT GC 400 BL00081:gyrB DNA gyrase subunit B 6263-6662
3gyrBR AAA TTT CTT CGA GCT GCT GGT