Table 1 Primer list
From: Genotyping of B. licheniformisbased on a novel multi-locus sequence typing (MLST) scheme
Primer | Sequence (5’-3’) | Amplicon size (bp) | Target gene (s) | Target (from-to bp)in NC_00627 |
|---|---|---|---|---|
adk1L | GGT AAA GGG ACA CAG GCT GA | 518 | BL01030: adk ;adenylate kinase | 144018-144535 |
adk1R | TCG AGT AAA GGC TGG GTT TG | |||
ccpA2L | TAT GAT GTA GCA CGC GAA GC | 604 | BL00444: ccpA; transcriptional regulator (compl) | 3007794-3008397 |
ccpA4R | TAT CCC CAA GCG CTC TTT TA | |||
recF2L | ACG GTT CTG TTC CCA TTC AG | 603 | BL00079: recF; recombination protein F | 3791-4393 |
recF2R | CAT CAC GGC CAT TGA CAT AG | |||
sucC2L | GGG TCC CGA CGG CCA ACA AA | 595 | BL01285: sucC; succinyl-CoA synthetase, subunit beta | 1786001-1786595 |
sucC2R | GGC CGG TTC CCC TCC GTA GT | |||
rpoB-f | AGG TCA ACT AGT TCA GTA TGG ACG[1] | 580 | BL02798: rpoB; DNA-directed RNA polymerase, subunit beta | 120955-121534 |
rpoB-r | AAG AAC CGT AAC CGG CAA CTT[1] | |||
spo0A1L | GAA GTG CTT GGT GTC GCA TA | 627 | BL01518: spo0A; response regulator | 2512117-2512743 |
spo0A1R | TGT GTA GCC GAA AAG TGA CG | |||
pyrE2L | AAA TCA AAG CGG TTT TCC TG | 575 | BL02280: pyrE; orotate phosphoribosyltransferase | 1734961-1735535 |
pyrE1R | AGG ATC CGC TTT CCA TTC TT | |||
glpT1L | CTT ACG GGC TGA GCA AGT TC | 593 | BL00185: glpT; glycerol-3-phosphate permease | 4094701-4095293 |
glpT1R | CAC GAA AAT GTT GGC AAG TG | |||
3gyrBF | ATC GTT GAG GGT GAC TCT GC | 400 | BL00081:gyrB DNA gyrase subunit B | 6263-6662 |
3gyrBR | AAA TTT CTT CGA GCT GCT GGT |