Primer | Function* | Sequence (5' to 3') |
---|---|---|
TDC11 RT1 | tyrS probe amplification (F) tyrS probe amplification (R) | TCAATTACAGATCGGTGGGG ACTTACCATCGAATGCATCAAATG |
TRNA2B(2) TRNA(P) TyrS prom (F) tyrS prom (R) | mRNA-C quantification (F) mRNA-C quantification (R) mRNA-L quantification (F) mRNA-L quantification (R) | CGTAAATTAGAAGGGCCAGAGGCAG GATCAAGCCAGATTGCGCCACCTGCAG AACAGGCAATGATCAAAACGAAGTA CATAGGCTCCTAAAATGTAATTCGC |
TYR2 | PtyrS mapping (R) | ACTTCGTTTTGATCATTGCCTG |
TDC123 TDC130 | PtyrSΔ cloning and sequencing (F) PtyrSΔ cloning and sequencing (R) | AAAACTTCCCATATGCATTGTAACG CTGCAGCATTTTATATGTTTTGTAGTAA |
TYS (F) TYS(R) | tyrS overexpression (F) tyrS overexpression (R) | ATGGGTGGTGGATTTGCTAATATTATCGATGAATTAACTT TTGGAAGTATAAATTTTCATCAACTACTTTGGCCAAAAAG |