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Table 2 Oligonucleotides used in this study

From: The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of Enterococcus duransis transcriptionally regulated by tyrosine concentration and extracellular pH

Primer

Function*

Sequence (5' to 3')

TDC11

RT1

tyrS probe amplification (F)

tyrS probe amplification (R)

TCAATTACAGATCGGTGGGG

ACTTACCATCGAATGCATCAAATG

TRNA2B(2)

TRNA(P)

TyrS prom (F)

tyrS prom (R)

mRNA-C quantification (F)

mRNA-C quantification (R)

mRNA-L quantification (F)

mRNA-L quantification (R)

CGTAAATTAGAAGGGCCAGAGGCAG

GATCAAGCCAGATTGCGCCACCTGCAG

AACAGGCAATGATCAAAACGAAGTA

CATAGGCTCCTAAAATGTAATTCGC

TYR2

PtyrS mapping (R)

ACTTCGTTTTGATCATTGCCTG

TDC123

TDC130

PtyrSΔ cloning and sequencing (F)

PtyrSΔ cloning and sequencing (R)

AAAACTTCCCATATGCATTGTAACG

CTGCAGCATTTTATATGTTTTGTAGTAA

TYS (F)

TYS(R)

tyrS overexpression (F)

tyrS overexpression (R)

ATGGGTGGTGGATTTGCTAATATTATCGATGAATTAACTT

TTGGAAGTATAAATTTTCATCAACTACTTTGGCCAAAAAG

  1. *F, forward; R, reverse