The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of Enterococcus duransis transcriptionally regulated by tyrosine concentration and extracellular pH

Table 1 Strains and plasmids used in this study

Strain/Plasmid	Characteristics *	Source
STRAINS
E. coli TOP10		Invitrogen
L. lactis NZ9000	Plasmid-free strain	[37, 38]
E. durans IPLA655	Isolated from artisanal cheese. Tyramine producer	IPLA Collection
PLASMIDS
pUC18	Amp^R	[39]
pNZ9530	Ery^R, nisR-nisK	[40]
pILORI4	Ery^R, lacZ promoterless gene	[41]
pNZcLIC	Cm^R, expression vector	[42]
pDA12	Amp^R, pUC18 with P_tyrS cloned in SmaI	This work
pDA15	Amp^R, pUC18 with P_tyrS^Δ cloned in SmaI	This work
pDA16	Ery^R, pILORI4 including P_tyrS^Δ -lacZ fusion	This work
pNZcTyrS	Cm^R, pNZcLIC including tyrS	This work

* Amp^R, ampicillin resistant; Ery^R, erythromycin resistant; Cm^R, chloramphenicol resistant

ISSN: 1471-2180