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Figure 3 | BMC Microbiology

Figure 3

From: The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of Enterococcus duransis transcriptionally regulated by tyrosine concentration and extracellular pH

Figure 3

Primary-sequence and structural model of the E. durans IPLA655 tyrS mRNA leader region upstream the start of the coding region. The specifier (UAC), the Tbox sequence, and other highly-conserved motifs typical of genes regulated by tRNA-mediated antitermination appear highlighted in boxes. Sequence between arrows can adopt two alternative mutually exclusive structure conformations: terminator and antiterminator (stabilized by the cognate tRNA in absence of tyrosine). A transcriptional fusion of the tyrS promoter and the leader region with a deletion of the TBox-Terminator region (PtyrSΔ) was made (dashed line) to probe the role of the Tbox in the mechanism of tyrosine sensing

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