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Table 1 Burkholderia (B.) mallei and B. pseudomallei strains

From: Rapid identification of Burkholderia mallei and Burkholderia pseudomalleiby intact cell Matrix-assisted Laser Desorption/Ionisation mass spectrometric typing

Bacteria Origin Country Year fliC fliP Motility
B. mallei       
ATCC 23344T Human China 1942 + + -
NCTC 120 unknown United Kingdom 1920 + + -
NCTC 10230 Horse Hungary 1961 + + -
NCTC 10247* Human Turkey 1960 + + -
NCTC 10260 Human Turkey 1949 + + -
M1* unknown unknown unknown + + -
M2 unknown unknown unknown + + -
Rotz7 (SVP) unknown unknown unknown + + -
32 unknown unknown unknown + + -
34 unknown unknown unknown + + -
235 unknown unknown unknown + + -
237 unknown unknown unknown + + -
242 unknown unknown unknown + + -
Bogor Horse Indonesia unknown + + -
Mukteswar Horse India unknown + + -
Zagreb Horse former-Yugoslavia unknown + + -
Dubai 7 Horse United Arab Emirates 2004 + + -
B. pseudomallei       
ATCC 23343T Human unknown <1957 + - +
EF 15660* unknown unknown unknown + - +
NCTC 1688* Rat Malaysia 1923 + - +
PITT 225A* Human Thailand 1986 + - +
PITT 521 Human Pakistan 1988 + - +
PITT 5691 unknown unknown unknown + - +
120107RR0019 Human Italy 2007 + - +
H05410-0490 Human Asia unknown + - +
03-04448 Human unknown unknown + - +
03-04450 unknown unknown unknown + - +
  1. T type strain.
  2. *Constituents of the reduced reference set dedicated for the discrimination of B. mallei and B. pseudomallei.
  3. Characteristics of Burkholderia (B.) mallei and B. pseudomallei strains used to establish the database for the identification and differentiation with MALDI-TOF mass spectrometry. Species identity was confirmed by real-time PCR assays targeting a sequence of the fliC gene that is specific for both species but does not discriminate B. mallei from B. pseudomallei. The real-time PCR assay targeting fliP is specific for B. mallei. Motility was also assessed as a phenotypic marker because B. pseudomallei is motile while B. mallei is not.