Advancement of the 10-species subgingival Zurich Biofilm model by examining different nutritional conditions and defining the structure of the in vitrobiofilms

Table 1 Growth media

Medium	Abbreviation	Reference	Use
mFUM, 4 mM Glucose	mFUM4		Growth medium for biofilms
mFUM 4 mM Glucose, iHS (50%)	iHS		Growth medium for biofilms
mFUM, 0.3% Glucose (30%), saliva (60%), iHS (10%)	SAL		Growth medium for biofilms
mFUM, 0.3% Glucose		[12]	Liquid precultures of S. oralis, S. anginosus, V. dispar¹, F. nucleatum, A. oris, P. intermedia, C. rectus²
Pg medium³		[29]	Liquid precultures of P. gingivalis
Spirochaetes medium		[30]	Precultures of T. denticola
Modified OMIZ-W68⁴		[31]	Precultures of T. forsythia

¹ addition of 1% lactic acid (v/v).
² addition of 0.1% sodium fumarate and 0.1% sodium formiate.
³ Brain heart infusion broth, supplemented with haemin (7.67 μM) and menadione (2.91 μM).
⁴ addition of lactose (2 g l^-1), caseinoglycomacropeptide (100 mg l^-1),N-acetylmuramic acid (50 mg l^-1), and N- acetylglucosamine (500 mg l^-1).

ISSN: 1471-2180