Thickness (A) and total counts (FISH/IF) (B) of biofilms grown for 64.5 h in SAL, mFUM4, and iHS growth medium. Thickness was determined by CLSM, total counts were calculated from the species specific quantification by visual microscopic counting following FISH- or IF from N=9 independent biofilms from three independent experiments. Thickness was determined at N=44 (iHS), N=61 (mFUM4) and N=57 (SAL) randomly selected measurement spots on the discs. The boxes represent the inter quartile range of the data points, the bar indicates the median. The whiskers cover the data points within the 1.5x inter quartile range. Dots are outliers within 1.5 and 3 box lengths outside the interquartile range. ** indicates the significantly higher thickness (p≤0.001) of iHS biofilms compared to biofilms of both SAL and mFUM4.