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Figure 4 | BMC Microbiology

Figure 4

From: Virulence of the Pseudomonas fluorescens clinical strain MFN1032 towards Dictyostelium discoideumand macrophages in relation with type III secretion system

Figure 4

Cytotoxic activity on macrophage J774A. 1. J774A.1 macrophages grown in 24-well plates for 20 h were infected with strains grown to an OD580nm of 1.0-1.5 (MOI of 5). The cytotoxicity was followed over a 4 h period by measuring LDH release using a cytotoxicity detection kit (Promega). Values are expressed as a mean concentration of LDH in the culture after 4 h of incubation. Data are mean values from three independent experiments.

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