Changes in human gut flora with age: an Indian familial study

BMC Microbiology

Table 1 Primers used for Real-Time PCR

Target organism	Primer	Sequence	PCR product (bp)
Clostridium coccoides-Eubacteria rectale group	ClEubF	CGGTACCTGACTAAGAAGC	429 [47]
	ClEubR	AGTTTYATTCTTGCGAACG
Prevotella	PrevF	CACCAAGGCGACGATCA	283 [19]
	PrevR	GGATAACGCCYGGACCT
Lactobacillus group	LacF	AGCAGTAGGGAATCTTCC	341 [48]
	LacR	ACACCGCTACACATGGAG
Bacteroides-Prevotella group	BacF	GAAGGTCCCCCACATTG	410 [49]
	BacR	CAATCGGAGTTCTTCGTG
Bifidobacterium	BifF	GCGTGCTTAACACATGCAAGTC	126 [50]
	BifR	CACCCGTTTCCAGGAGCTATT
Roseburia	RosF	TACTGCATTGGAAACTGTCG	230 [19]
	RosR	CGGCACCGAAGAGCAAT
All bacteria	27F	TCCTACGGGAGGCAGCAGT	316 [This study]
	343R	GACTACCAGGGTATCTAATCCTGTT

Legend: ClEub- Clostridium coccoides-Eubacteria rectale group specific primers, Prev- Prevotella genus specific primers, Lac- Lactobacillus genus specific primers, Bac-Prev- Bacteriodes-Prevotella specific primers, Bif- Bifidobacterium genus specific primers , Ros- Roseburia genus specific primers and All bacteria- universal primers for all bacteria.

ISSN: 1471-2180