The effect of training set on the classification of sequences from the honey bee gut visualized by a heat map. Unique sequences (4,480) were classified using the NBC trained on either RDP, GG, or SILVA (A), three custom databases including near full length honey bee-associated sequences RDP + bees, GG + bees, SILVA + bees (B), or the near full length honey bee-associated sequences alone (C). Family-level taxonomic designations are shown and where taxonomic classifications occur across all three datasets, these are highlighted in bold lettering. Where a classification is unique to one training set, this is highlighted in red font. The average bootstrap score resulting from the classification is provided for each taxonomic assignment.