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Table 2 A list of the markers selected to represent published DNA-based markers for molecular PCR detection or phylogenetic identification targeting Francisella

From: Increased knowledge of Francisellagenus diversity highlights the benefits of optimised DNA-based assays

Marker name/ Target gene Gene locus_taga Amplicon size (bp)a Genomic locationa Reference
01-16S FTT_r04, FTT_r07, FTT_r10 1139 1311156-2294, 1378275–9413, 1771610-2748 [17, 37, 38, 56]
02-16 s + ItS + 23 s FTT_r04, FTT_r07, FTT_r10 915 1311470-2371, 1378876–9490, 1771911-2825 [34]
03-16 s + ItS + 23 s FTT_r03-FTT_r04, FTT_r06-FTT_r07, FTT_r09-FTT_r10 948 1310519-1466, 1377638–8585, 1770973-1920 [34]
04-16 s + ItS + 23 s FTT_r03, FTT_r06, FTT_r09 925 1309613-10537, 1376732–7656, 1770067-991 [34]
05-aroA FTT_0588 650 608150-799 [18, 61]
06-atpA FTT_0062 634 62762-3395 [18, 61]
07-dnaA FTT_0001 618 303-920 [19]
08-fabH FTT_1373 1289 1418892-20155 [62]
09-fopA FTT_0583 886 599105-990 [19]
10-fopA FTT_0583 1068 599148-600215 [34]
11-fopA-in FTT_0583 404 599526-929 [15]
12-fopA-out FTT_0583 708 599428-600135 [15]
13-fopA FTT_0583 86 599767-852 [9, 16]
14-FtM19 FTT_1472c 250 1524132-381 [56, 58]
15-FtM19 FTT_1472c 316 1524066-381 [65]
16-FTT0376 FTT_0376 107 377718-824 [17]
17-FTT0523 FTT_0523 91 546620.712 [17]
18-groEL FTT_1696 803 1764659-5461 [34]
19-iglC FTT_1712c 84 1792514-597 [9, 16]
20-ISFtu2b FTT_1311 390 1335726-6115 [56, 59]
21-ISFtu2 FTT_0099c 97 103438-534c [9, 16]
22-lpnAb FTT_0901 407 909857-10263 [19, 37, 38, 56, 57]
23-lpnA FTT_0901 93 910211-301 [9, 16]
24-lpnB FTT_0904 252 911795-2046 [34]
25-mdh FTT_0535c 715 556932-7646 [63, 64]
26-mutS FTT_1499 495 1553224-3718 [19]
27-parC FTT_0396 643 397063-705 [18, 61]
28-pdpD FTT_1360c, FTT_1715c 136 1403503-638, 1796838-973 [56, 60]
29-pgm FTT_0414 650 425033-682 [18, 61]
30-prfB FTT_0191 376 207686-8061 [19]
31-putA FTT_1150c 415 1165411-825 [19]
32-rpoA FTT_0350, FTT_1442c 914 349619-50532 [64]
33-rpoB FTT_0144 262 156309-570 [34]
34-sdhA FTT_0074 223 75065-287 [34]
35-tpiA FTT_0080 484 83679-4162 [19]
36-tpiA FTT_0080 559 83657-4215 [18, 61]
37-trpE FTT_1802c 517 1888928-9444 [18, 61]
38-uup FTT_0445 645 459229-873 [18, 61]
  1. a Amplicon locus tag, length and location in genome of F. tularensis strain SCHU S4.
  2. b Primer sequence of primer Tuf1705 in marker 20-ISFtu2 and TUL-435 in marker 22-lpnA seem to be incorrectly specified in [56]. See [37] and [59] for the correct primer sequences.
  3. c Insertion element present in multiple copies in reference. Only first position and gene specified.
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BMC Microbiology

ISSN: 1471-2180

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