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Figure 1 | BMC Microbiology

Figure 1

From: Increased knowledge of Francisellagenus diversity highlights the benefits of optimised DNA-based assays

Figure 1

Overview of primer specificity. Weighted score of primer specificity calculated with penalties for mismatches and gaps, where zero indicates a perfect match. The first column of each marker represents the forward primer score and the second represents the reverse primer score. The score was calculated with PrimerProspector as follows: 3’ mismatch, 1 penalty per mismatch (length of 3’ region was set to 5), non-3’ mismatch, (0.4 penalty per mismatch), last base mismatch (penalty 3 per mismatch), non 3’ gap (penalty 1 per gap) and 3’ gap (penalty 3 per gap).

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