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Figure 1 | BMC Microbiology

Figure 1

From: A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensisinfection

Figure 1

Comparison of differentially expressed genes using microarray and RT-qPCR techniques. RT-qPCR was used to verify the differential expression of randomly selected genes (n = 27) by uninfected C57BL/6 and CBA macrophages (A), by L. amazonensis-infected C57BL/6 macrophages in comparison to uninfected cells (n = 7) (B), and by L. amazonensis-infected CBA macrophages in comparison to uninfected cells (n = 2) (C). Figure 1 (A-C) depicts only genes that were successfully verified using RT-qPCR. Resulting comparison values are expressed as mean values of log2 ± SE from two independent experiments in comparison (A), and three independent experiments in comparisons (B) and (C), all performed in duplicate. The nonparametric Mann-Whitney test was used for comparison between uninfected cells, and Stouffer method [29] was used to integrate the results from independent microarray and RT-qPCR analyses to determine significant differences between infected and uninfected cells (level of significance, p ≤ 0.05)

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