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Table 2 Strains and plasmids used in this study

From: Characterization of EssB, a protein required for secretion of ESAT-6 like proteins in Staphylococcus aureus

Strains Description Reference
RN4220 S. aureus sau1 hsdR laboratory strain used for passaging plasmid DNA [34, 35]
USA300 Community-acquired methicillin resistant S. aureus NARSA repository [36]
essB USA300 carrying an internal deletion of essB This study
DH5α E. coli K12 fhuA2 Δ (argF-lacZ)U169 phoA glnV44 Φ80 Δ (lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 for cloning Our collection
BL21(DE3) E. coli B F- dcm ompT hsdS (rB-mB-) gal for recombinant protein production Our collection
Plasmids Description Reference
pWWW412 pOS1 derivative carrying the constitutive hprK promoter, CmR [33]
pKOR1 temperature sensitive plasmid for allelic replacement, CmR [32]
pET15b Vector carrying an N-terminal hexa-histidine repeat followed by a thrombin cleavage site and T7 transcription start; AmpR Novagen
pGEX-2TK Vector carrying GST followed by thrombin cleavage and phosphorylation sites, and a taq promoter; AmpR GE Healthcare
p essB pWWW412 expressing wild-type essB codons 1-444 This study
p essB N pWWW412 expressing codons 1–223 of essB This study
p essB NM pWWW412 expressing codons 1–252 of essB This study
p essB C pWWW412 expressing codons 253–444 of essB This study
p essB CM pWWW412 expressing codons 220–444 of essB This study
p essB ΔM pWWW412 expressing essB lacking codons 224-252 This study
p his - essB pET15b expressing histidine tagged essB codons 1-444 This study
p his - essB NM pET15b expressing histidine tagged essB codons 1- 252 This study
p his - essB CM pET15b expressing histidine tagged essB codons 220- 444 This study
p his - essB ΔM pET15b expressing histidine tagged essB lacking codons 224-252 This study
p gst - essB N pGEX-2TK expressing GST fused to codons 1–223 of essB This study
p gst - essB C pGEX-2TK expressing GST fused to codons 253–444 of essB This study