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Table 1 Differential Immunity profiles of each LES phage in PAO1

From: Differential infection properties of three inducible prophages from an epidemic strain of Pseudomonas aeruginosa

Efficiency of plating values

φ2

φ3

φ4

PAO1 naive host

1.0

1.0

1.0

Single φ2 lysogen

< 1x10 -9

< 1x10 -9

0.017

Single φ3 lysogen

0.91

< 1x10 -9

0.37

Single φ4 lysogen

1.09

0.94

3.3x10 -9

  1. Immunity profiles of each LES phage were determined by plaque assay. Phage dilution series were spotted onto non-Lysogenic PAO1 and PLPL lawns. Efficiency of plating = the ratio of plaques observed (at the appropriate phage dilution) on the most permissive host (non-lysogenic PAO1)/plaques observed on assay host (PLPL harbouring LESφ2, LESφ3 or LESφ4 prophages). If no plaques were observed when neat phage suspensions of 1010 p.f.u ml-1 were used, an eop value < 1x10-9 was recorded.
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BMC Microbiology

ISSN: 1471-2180

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