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Table 1 Protein detection using shotgun (single-plex) and iTRAQ labelled 4-plex 2D-HPLC-MS/MS and relative changes in protein expression levels

From: Proteomic analysis of Clostridium thermocellum core metabolism: relative protein expression profiles and growth phase-dependent changes in protein expression

Core metabolic protein categories Total genes Proteins detected Changes in protein levels (Stat/Exp)
  1-Plex 4-Plex Total V diff  ≥ 0.5
     Increased Decreased
Non-catalytic cellulosomal proteins 8 5 6 7 0 0
Cellulosomal glycosidase 73 29 26 31 2 1
Non-cellulosomal glycosidases 35 17 13 19 3 0
RsgI-like σ-factors and anti-σI factors 9 3 2 3 0 0
Cello-oligosaccharide ABC transporters 14 9 8 10 2 1
Glycolysis 20 15 15 15 3 1
Pentose phosphate pathway 6 4 3 5 1 0
Energy storage 13 11 11 13 3 0
Pyruvate formation from phosphoenolpyruvate 8 8 8 8 0 2
End-product synthesis from pyruvate 49 39 38 41 12 0
Energy generation 17 14 14 14 2 1
Total 252 154 144 166 28 6
  1. Core metabolic proteins were classified into functional categories. The total number of protein encoding genes in each category and the number of corresponding proteins detected are provided. The number of proteins that changed during transition from exponential to stationary phase were listed only when their vector difference (V diff ) was greater than 0.5. Proteins detected can be viewed inAdditional files 3and 4.