Skip to main content

Table 1 Protein detection using shotgun (single-plex) and iTRAQ labelled 4-plex 2D-HPLC-MS/MS and relative changes in protein expression levels

From: Proteomic analysis of Clostridium thermocellum core metabolism: relative protein expression profiles and growth phase-dependent changes in protein expression

Core metabolic protein categories

Total genes

Proteins detected

Changes in protein levels (Stat/Exp)

 

1-Plex

4-Plex

Total

V diff  ≥ 0.5

    

Increased

Decreased

Non-catalytic cellulosomal proteins

8

5

6

7

0

0

Cellulosomal glycosidase

73

29

26

31

2

1

Non-cellulosomal glycosidases

35

17

13

19

3

0

RsgI-like σ-factors and anti-σI factors

9

3

2

3

0

0

Cello-oligosaccharide ABC transporters

14

9

8

10

2

1

Glycolysis

20

15

15

15

3

1

Pentose phosphate pathway

6

4

3

5

1

0

Energy storage

13

11

11

13

3

0

Pyruvate formation from phosphoenolpyruvate

8

8

8

8

0

2

End-product synthesis from pyruvate

49

39

38

41

12

0

Energy generation

17

14

14

14

2

1

Total

252

154

144

166

28

6

  1. Core metabolic proteins were classified into functional categories. The total number of protein encoding genes in each category and the number of corresponding proteins detected are provided. The number of proteins that changed during transition from exponential to stationary phase were listed only when their vector difference (V diff ) was greater than 0.5. Proteins detected can be viewed inAdditional files 3and 4.