Experiments | Basal medium | Carbon/e- donor | e- acceptor/substrate added | Headspace gas | Comments |
---|---|---|---|---|---|
Pyruvate fermentation | DCB1*, vitamins | Pyruvate, 20 mM | Â | N2, 95% CO2, 5% | Reference culture for microarray |
Fe(III) reduction | CBF**, vitamins | Lactate, 20 mM | Ferric citrate, 50 mM or Ferric oxide, 50 mM | N2, 95% CO2, 5% | Ferric citrate for microarray Ferric oxide for growth study only |
Se(VI) reduction | DCB1, vitamins | Pyruvate, 20 mM | Sodium selenate, 1 mM | N2, 95% CO2, 5% | For microarray |
U(VI) reduction | DCB1, vitamins | Pyruvate, 20 mM | Uranyl acetate, 0.5 mM | N2, 95% CO2, 5% | For microarray |
As(V) reduction | DCB1, vitamins | Pyruvate, 20 mM | Sodium arsenate, 1 mM | N2, 95% CO2, 5% | For growth study only |
Nitrate reduction | CBF, vitamins | Lactate, 20 mM | Potassium nitrate, 10 mM | N2, 95% CO2, 5% | For microarray |
DMSO/TMAO reduction | DCB1, vitamins | Lactate, 20 mM | DMSO, 5 mM or TMAO, 5 mM | N2, 95% CO2, 5% | For growth study only |
3-Cl-4-OH-BA dechlorination | DCB1, vitamins | Pyruvate, 20 mM or Lactate, 20 mM | 3-chloro-4-hydroxybenzoate 1 mM or 50 μM for growth | N2, 95% CO2, 5% | Pyruvate for microarray & northern blot Lactate for growth study |
3,5-DCP dechlorination | DCB1, vitamins | Pyruvate, 20 mM or Lactate, 20 mM | 3,5-dichlorophenol 1 mM or 50 μM for growth | N2, 95% CO2, 5% | Pyruvate for microarray & northern blot Lactate for growth study |
o-BP debromination | DCB1, vitamins | Pyruvate, 20 mM or Lactate, 20 mM | ortho-bromophenol 1 mM or 50 μM for growth | N2, 95% CO2, 5% | Pyruvate for microarray & northern blot Lactate for growth study |
Oxygen effect | DCB1, vitamins | Pyruvate, 20 mM | Â | N2, 95% CO2, 5% | Exposure to air for 3 hours after fermentative cell growth |
N2 fixation | DCB1, vitamins | Pyruvate, 20 mM | Â | N2, 95% CO2, 5% | NH4+ omitted from DCB1 Gas replenished every 12 h |
CO2 fixation | DCB1, vitamins | Â | Â | CO, CO2 N2, H2 | Details in Figure 3 |