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Table 2 Culture conditions of D. hafniense DCB-2

From: Genome sequence of Desulfitobacterium hafnienseDCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction

Experiments

Basal medium

Carbon/e- donor

e- acceptor/substrate added

Headspace gas

Comments

Pyruvate fermentation

DCB1*, vitamins

Pyruvate, 20 mM

 

N2, 95%

CO2, 5%

Reference culture for microarray

Fe(III) reduction

CBF**, vitamins

Lactate, 20 mM

Ferric citrate, 50 mM or

Ferric oxide, 50 mM

N2, 95%

CO2, 5%

Ferric citrate for microarray

Ferric oxide for growth study only

Se(VI) reduction

DCB1, vitamins

Pyruvate, 20 mM

Sodium selenate, 1 mM

N2, 95%

CO2, 5%

For microarray

U(VI) reduction

DCB1, vitamins

Pyruvate, 20 mM

Uranyl acetate, 0.5 mM

N2, 95%

CO2, 5%

For microarray

As(V) reduction

DCB1, vitamins

Pyruvate, 20 mM

Sodium arsenate, 1 mM

N2, 95%

CO2, 5%

For growth study only

Nitrate reduction

CBF, vitamins

Lactate, 20 mM

Potassium nitrate, 10 mM

N2, 95%

CO2, 5%

For microarray

DMSO/TMAO

reduction

DCB1, vitamins

Lactate, 20 mM

DMSO, 5 mM or

TMAO, 5 mM

N2, 95%

CO2, 5%

For growth study only

3-Cl-4-OH-BA dechlorination

DCB1, vitamins

Pyruvate, 20 mM

or Lactate, 20 mM

3-chloro-4-hydroxybenzoate

1 mM or 50 μM for growth

N2, 95%

CO2, 5%

Pyruvate for microarray & northern blot

Lactate for growth study

3,5-DCP dechlorination

DCB1, vitamins

Pyruvate, 20 mM

or Lactate, 20 mM

3,5-dichlorophenol

1 mM or 50 μM for growth

N2, 95%

CO2, 5%

Pyruvate for microarray & northern blot

Lactate for growth study

o-BP debromination

DCB1, vitamins

Pyruvate, 20 mM

or Lactate, 20 mM

ortho-bromophenol

1 mM or 50 μM for growth

N2, 95%

CO2, 5%

Pyruvate for microarray & northern blot

Lactate for growth study

Oxygen effect

DCB1, vitamins

Pyruvate, 20 mM

 

N2, 95%

CO2, 5%

Exposure to air for 3 hours after fermentative cell growth

N2 fixation

DCB1, vitamins

Pyruvate, 20 mM

 

N2, 95%

CO2, 5%

NH4+ omitted from DCB1

Gas replenished every 12 h

CO2 fixation

DCB1, vitamins

  

CO, CO2

N2, H2

Details in Figure 3

  1. DCB1*, modified DCB1 medium [61]
  2. CBF**, modified CBF medium [32]