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Table 2 Culture conditions of D. hafniense DCB-2

From: Genome sequence of Desulfitobacterium hafnienseDCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction

Experiments Basal medium Carbon/e- donor e- acceptor/substrate added Headspace gas Comments
Pyruvate fermentation DCB1*, vitamins Pyruvate, 20 mM   N2, 95%
CO2, 5%
Reference culture for microarray
Fe(III) reduction CBF**, vitamins Lactate, 20 mM Ferric citrate, 50 mM or
Ferric oxide, 50 mM
N2, 95%
CO2, 5%
Ferric citrate for microarray
Ferric oxide for growth study only
Se(VI) reduction DCB1, vitamins Pyruvate, 20 mM Sodium selenate, 1 mM N2, 95%
CO2, 5%
For microarray
U(VI) reduction DCB1, vitamins Pyruvate, 20 mM Uranyl acetate, 0.5 mM N2, 95%
CO2, 5%
For microarray
As(V) reduction DCB1, vitamins Pyruvate, 20 mM Sodium arsenate, 1 mM N2, 95%
CO2, 5%
For growth study only
Nitrate reduction CBF, vitamins Lactate, 20 mM Potassium nitrate, 10 mM N2, 95%
CO2, 5%
For microarray
DMSO/TMAO
reduction
DCB1, vitamins Lactate, 20 mM DMSO, 5 mM or
TMAO, 5 mM
N2, 95%
CO2, 5%
For growth study only
3-Cl-4-OH-BA dechlorination DCB1, vitamins Pyruvate, 20 mM
or Lactate, 20 mM
3-chloro-4-hydroxybenzoate
1 mM or 50 μM for growth
N2, 95%
CO2, 5%
Pyruvate for microarray & northern blot
Lactate for growth study
3,5-DCP dechlorination DCB1, vitamins Pyruvate, 20 mM
or Lactate, 20 mM
3,5-dichlorophenol
1 mM or 50 μM for growth
N2, 95%
CO2, 5%
Pyruvate for microarray & northern blot
Lactate for growth study
o-BP debromination DCB1, vitamins Pyruvate, 20 mM
or Lactate, 20 mM
ortho-bromophenol
1 mM or 50 μM for growth
N2, 95%
CO2, 5%
Pyruvate for microarray & northern blot
Lactate for growth study
Oxygen effect DCB1, vitamins Pyruvate, 20 mM   N2, 95%
CO2, 5%
Exposure to air for 3 hours after fermentative cell growth
N2 fixation DCB1, vitamins Pyruvate, 20 mM   N2, 95%
CO2, 5%
NH4+ omitted from DCB1
Gas replenished every 12 h
CO2 fixation DCB1, vitamins    CO, CO2
N2, H2
Details in Figure 3
  1. DCB1*, modified DCB1 medium [61]
  2. CBF**, modified CBF medium [32]