Carbon metabolic pathways of D. hafniense DCB-2. The pathways were constructed based on the presence or absence of key metabolic genes in D. hafniense DCB-2. The acetyl-CoA degradation and related genes are shown in more detail (boxed). Enzymes for the numbered reactions in figure are listed below with their potential genes; 1. pyruvate kinase; Dhaf_2755. 2. phosphoenolpyruvate synthase; Dhaf_1117, Dhaf_1622, Dhaf_3294. 3. pyruvate, phosphate dikinase; Dhaf_1046, Dhaf_4240, Dhaf_4251. 4. D-lactate dehydrogenase (cytochrome); Dhaf_3228, Dhaf_4382. 5. L-lactate dehydrogenase; Dhaf_1965. 6. PEP carboxykinase; Dhaf_1134. 7. malate dehydrogenase (NADP+); Dhaf_0902, Dhaf_3085. 8. pyruvate carboxyltransferase; Dhaf_1012, Dhaf_1059. 9. pyruvate formate-lyase; Dhaf_0366, Dhaf_1246, Dhaf_4905. 10. pyruvate flavodoxin/ferredoxin oxidoreductase; Dhaf_0054, Dhaf_4766. 11a. acetate-CoA ligase; Dhaf_0467. 11b. acetyl-CoA hydrolase/transferase; Dhaf_0603, Dhaf_2858, Dhaf_4529. 12. aldehyde dehydrogenase (NAD+); Dhaf_2181. 13. acetaldehyde dehydrogenase (acetylating); Dhaf_2180. 14. malate dehydrogenase; Dhaf_1799, Dhaf_4412. 15. citrate lyase; Dhaf_4206. 16. succinate-CoA ligase (ADP-forming); Dhaf_0192, Dhaf_2066. 17. alcohol dehydrogenase; Dhaf_2180, Dhaf_0588. 18. succinate dehydrogenase; Dhaf_0743-0745. 19. fumarase; Dhaf_4397. 20. citrate synthase; Dhaf_0903. 21. isocitrate dehydrogenase (NADP+); Dhaf_1523. 22. hydrogen:quinone oxidoreductase; Dhaf_2742. 23. hydrogenase (ferredoxin); Dhaf_0805, Dhaf_3270, Dhaf_3368. 24. formate dehydrogenase; Dhaf_1398, Dhaf_1509, Dhaf_4271. 25. aconitase; Dhaf_1133. 26. tryptophanase; Dhaf_1324, Dhaf_2460.