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Table 1 Bacterial strains and plasmids used in this study

From: Role of trehalose in heat and desiccation tolerance in the soil bacterium Rhizobium etli

Strain or plasmid Relevant genotype and/or description Source or reference
R. etli strains   
 CFN 42 Wild type [29]
 CE3 Spontaneous Smr mutant of R, etli CFN 42 Nalr [31]
 CMS310 R. etli CE3 otsAch::ΩNalrSmrSpcr This study
E. coli strain   
 DH5α supE44 Δ (lac)U169 ϕ80d lacZΔM15 hsdR17 recA1 endA1 gyrA96 thi-1 relA1; host for DNA manipulations [35]
Plasmids   
 pSK(-) Cloning vector; Apr Stratagene
 pUC19-301 Cloning vector, Apr J. Onofre, Personal Communication
 pHP45Ω pBR322 derivative carrying the Ω cassette; AprSmrSpr [36]
 pRK600 Helper plasmid; Cmrtra [37]
 pJQ200-SK Suicide vector; Gmrmobsac [38]
 pMotsA1 4.2-kb blunt fragment from R. etli CE3 genome (containing frk, otsAch, pgi) cloned into pUC19301 in Eco RV; Apr This study
 pMotsA4 4,1-kb Bgl II-Xba I fragment from pMotsA1 cloned into pSK in Bam HI-Xba I; Apr This study
 pMotsA5 pMotsA4 derivative containing an Bgl II recognition site within otsAch; Apr This study
 pMotsA6 pMotsA5 derivative with Ω casete within otsAch; AprSmrSpcr This study
 pMotsA7 6.1-kb Apa I-Xba I fragment from pMotsA6 (containing frk,otsAch, pgi) cloned into pJQ200-SK; GmrSmrSpcr This study