Growth and accumulation of trehalose by R. etli in response to high temperature and salinity stress. Cells were grown in mannitol minimal medium B- at 28°C and 35°C, with 0.0 and 0.2 M NaCl, up to early stationary phase. Trehalose content was measured colorimetrically as described in Materials and Methods. For each determination, a growth curve under the same condition used to measure trehalose accumulation is shown. Histograms representing trehalose accumulation are shown above the sampling time. The trehalose values are the mean of three replicas of each condition in two independent experiments ± SD (standard deviation).