Figure 6From: Illegitimate recombination: An efficient method for random mutagenesis in Mycobacterium avium subsp. hominissuisIntracellular survival of mutants compared to WT in human monocytes. Human blood monocytes (1.0x106) from healthy volunteers were infected (MOI 10) with mutants and WT. Intracellular bacteria were quantified after 4 hour of infection, and after 1, 2, & 4 days. The monocytes were lysed in 1 ml of sterile water and 100 μl of 1:500 dilution in sterile water of sample were plated on Middlebrook agar plates supplemented with ADC for CFU counting. A: WT and mutant MAV_4334; B: WT and mutant MAV_5106; C: WT and mutant MAV_1778; D: WT and mutant MAV_3128. Statistical analysis was done using a two tailed, paired Student’s t test. When compared to wild-type a P < 0.05 was considered significant (*) and a P < 0.01 very significant (**).Back to article page