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Figure 3 | BMC Microbiology

Figure 3

From: Tigecycline challenge triggers sRNA production in Salmonella enterica serovar Typhimurium

Figure 3

Northern blots for sYJ5, sYJ20 (SroA), sYJ75 and sYJ118 A) in SL1344 challenged with half the MIC of tetracycline, B) ciprofloxacin or ampicillin, and the four sRNAs level in E. coli and K. pneumoniae challenged with half the MIC of tigecycline. A) Lanes with - were loaded with control samples; lanes with + were loaded with total RNA extracted from cells challenged with half the MIC of tetracycline. This image is composite from different experiments. B) Lanes marked by - were loaded with control total RNA extracted from S. Typhimurium. Lanes marked as C were loaded with the total RNA extracted from S. Typhimurium that was challenged with half the MIC of ampicillin (1 µg/ml). Lanes marked by K- were loaded with the control total RNA extracted from K. pneumoniae. Lanes marked as K + were loaded with the total RNA extracted from K. pneumoniae that was challenged with half the MIC of tigecycline. Lanes marked as E- were loaded with the control total RNA extracted from E. coli. Lanes marked as E + were loaded with the total RNA extracted from E. coli that was challenged with half the MIC of tigecycline. Probe sequences were checked for 100% identity match in K. pneumoniae and E. coli prior to use.

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