|
Progesterone concentration (mM)
|
Average diameter of colonies incubated at 25°C (cm)a,b,c
|
Average diameter of colonies incubated at 35°C (cm)a,b,c
|
|---|
|
0
|
2.40 ± 0.18
|
1.47 ± 0.13
|
|
0.010
|
2.35 ± 0.10
|
1.33 ± 0.11
|
|
0.050
|
2.10 ± 0.11*
|
no growth
|
|
0.125
|
1.78 ± 0.07*
|
no growth
|
|
0.250
|
1.47 ± 0.16*
|
no growth
|
|
0.500
|
1.22 ± 0.11*
|
no growth
|
- This table shows the colony diameter attained after conidia were inoculated at 25°C and 35°C in a modification of medium M agar plates with different concentrations of added progesterone. No growth was observed at concentrations of progesterone of 0.05 mM or above, at 35°C while conidia incubated at 25°C germinated and showed growth at all concentrations of progesterone tested. The data represents the average diameter ± one std deviation of 6 independent experiments.
- a The cultures were incubated at the desired temperature for 20 days.
- b All cultures were inoculated with 5μl of a suspension containing 106/μl conidia.
- c The values given are the average of 6 independent determinations.
- * The values marked with an asterisk are significantly different from the values where no progesterone was added to the medium.
