Characterization and ligand identification of a membrane progesterone receptor in fungi: existence of a novel PAQR in Sporothrix schenckii

Table 1 Effects of Progesterone on S. schenckii yeast and mycelium growth from conidia

Progesterone concentration (mM)	Average diameter of colonies incubated at 25°C (cm)^a,b,c	Average diameter of colonies incubated at 35°C (cm)^a,b,c
0	2.40 ± 0.18	1.47 ± 0.13
0.010	2.35 ± 0.10	1.33 ± 0.11
0.050	2.10 ± 0.11*	no growth
0.125	1.78 ± 0.07*	no growth
0.250	1.47 ± 0.16*	no growth
0.500	1.22 ± 0.11*	no growth

This table shows the colony diameter attained after conidia were inoculated at 25°C and 35°C in a modification of medium M agar plates with different concentrations of added progesterone. No growth was observed at concentrations of progesterone of 0.05 mM or above, at 35°C while conidia incubated at 25°C germinated and showed growth at all concentrations of progesterone tested. The data represents the average diameter ± one std deviation of 6 independent experiments.
^a The cultures were incubated at the desired temperature for 20 days.
^b All cultures were inoculated with 5μl of a suspension containing 10⁶/μl conidia.
^c The values given are the average of 6 independent determinations.
* The values marked with an asterisk are significantly different from the values where no progesterone was added to the medium.

ISSN: 1471-2180