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Table 3 Strains and plasmids used in this study

From: Physiology of deletion mutants in the anaerobic β-myrcene degradation pathway in Castellaniella defragrans

Strains or plasmids Genotype, markers and further characteristics Source/reference
Strains   
 E. coli   
 S17-1 Thi, pro, hsdR, recA with RP4-2[Tc::Mu-Km::Tn7] [63]
 One Shot®Top10 F- mcr A Δ(mrr-hsd RMS-mcr BC) φ80lac ZΔM15 Δlac X74 rec A1 ara D139 Δ(araleu) 7697 gal U gal K rps L (StrR) end A1 nup G Invitrogen
 C. defragrans   
 65Phen Wild type [40]
 65Phen-RIFa RaR This study
 65Phen-RIF Δldib RaR, Δldi This study
 65Phen-RIF Δldi compc RaR, Δldi, pBBR1MCS-4ldi This study
 65Phen-RIF ΔgeoAd RaR, ΔgeoA This study
 65Phen-RIF ΔgeoA compe RaR, ΔgeoA, pBBR1MCS-2geoA This study
Plasmids   
 pCR4-TOPO AmR, KmR, lac Invitrogen
 pK19mobsacB KmR, sacB modified from B. subtilis, lac [64]
 pK19mobsacBΔldi KmR, sacB modified from B. subtilis, lac Zα, ORF25, ORF27 This study
 pK19mobsacBΔgeoA KmR, sacB modified from B. subtilis, lac Zα, ORF29-30, ORF32 This study
 pBBR1MCS-4 AmR , mob, lac [65]
 pBBR1MCS-4ldi AmR, mob, lac Zα, ldi This study
 pBBR1MCS-2 KmR, mob, lac [65]
 pBBR1MCS-2geoA KmR, mob, lac Zα, geoA This study
  1. a abbreviated in course of the text to C. defragrans RIF, b abbreviated to C. defragrans Δldi, c abbreviated to C. defragrans Δldi comp, d abbreviated to C. defragrans ΔgeoA, e abbreviated to C. defragrans ΔgeoA comp.