Table 3 Strains and plasmids used in this study

Strains

 E. coli

 S17-1

Thi, pro, hsdR, recA with RP4-2[Tc::Mu-Km::Tn7]

[63]

 One Shot®Top10

F- mcr A Δ(mrr-hsd RMS-mcr BC) φ80lac ZΔM15 Δlac X74 rec A1 ara D139 Δ(araleu) 7697 gal U gal K rps L (Str^R) end A1 nup G

Invitrogen

 C. defragrans

 65Phen

Wild type

[40]

 65Phen-RIF^a

Ra^R

This study

 65Phen-RIF Δldi^b

Ra^R, Δldi

This study

 65Phen-RIF Δldi comp^c

Ra^R, Δldi, pBBR1MCS-4ldi

This study

 65Phen-RIF ΔgeoA^d

Ra^R, ΔgeoA

This study

 65Phen-RIF ΔgeoA comp^e

Ra^R, ΔgeoA, pBBR1MCS-2geoA

This study

Plasmids

 pCR4-TOPO

Am^R, Km^R, lac Zα

Invitrogen

 pK19mobsacB

Km^R, sacB modified from B. subtilis, lac Zα

[64]

 pK19mobsacBΔldi

Km^R, sacB modified from B. subtilis, lac Zα, ORF25, ORF27

This study

 pK19mobsacBΔgeoA

Km^R, sacB modified from B. subtilis, lac Zα, ORF29-30, ORF32

This study

 pBBR1MCS-4

Am^R , mob, lac Zα

[65]

 pBBR1MCS-4ldi

Am^R, mob, lac Zα, ldi

This study

 pBBR1MCS-2

Km^R, mob, lac Zα

[65]

 pBBR1MCS-2geoA

Km^R, mob, lac Zα, geoA

This study