Table 4 Oligonucleotide primers used in qRT-PCR with B. fragilis and B. thetaiotaomicron
Primer | Sequence |
|---|---|
qBfp1_F | TTTAACAAGAAGCGGTGAACAAAGAA |
qBfp1_R | TGCAATAGGAATACAACCCGCATAAT |
qBfp2_F | CTACAAAGATAAAGCCACGGGAGCTA |
qBfp2_R | TCTGTCTCCTCCCATAAAAACAGGTC |
qBfp3_F | GAGGTTGTAAAAACGACACCAGCAAT |
qBfp3_R | TGAGTATGCATAAATAGGTGCGGTTC |
qBfp4_F | TCGTAGTGGGCAGTCAGGTTACTACA |
qBfp4_R | ACTCTCCCAAACCATAGAATCCCAAT |
q16S_Bf_F | GCGCACGGGTGAGTAACACGTAT |
q16S_Bf_R | CGTTTACTGTGTGGACTACCAGG |
qBtpA_F | CGTCTTCTACCCCTTGTTTGAGATGT |
qBtpA_R | TTAAGTGACACGCTTCAATATCAGGAA |
qBtpC_F | GTGCTGTTATTTCAATAGCACAGATT |
qBtpC_R | TCTAGTTGTTTCAGAGGAAGGAGTTT |
qBtpB_F | TGGTATAAAAATAGATTGGGAAGCAT |
qBtpB_R | GGATGAGTACCAGAAAGGTCATAAAT |
qBtpZ_F | AATTGTGGTAATATTCAAAAATGGAG |
qBtpZ_R | AATATGCATTACTGCTAGAAGATTCG |
q16S_Bt_F | TCACTGGACTGCAACTGACACTGAT |
q16S_Bt_R | ACTCCCCAGGTGGAATACTTAATGCT |