Table 2 Primers for anaerobic hydrocarbon degradation genes detection
Primer Set | Forward (F) and Reverse (R) Oligonucleotide Primer Sequences | Expected amplicon size (bp) | Reference |
|---|---|---|---|
SP9/ASP1 (bamA) | F: 5`-CAG TAC AAY TCC TAC ACV ACB G-3` | ~300 | [20] |
R: 5`-C MAT GCC GAT YTC CTG RC-3` | |||
assA2F/R (assA) | F: 5’-YAT GWA CTG GCA CGG MCA-3’ | 440 | Aitken et al., unpublished observations |
R: 5’-GCR TTT TCM ACC CAK GTA-3’ | |||
7772 F/8546R (bssA) | F: 5’-GAC ATG ACC GAC GCS ATY CT-3’ | ~794 | [22] |
R: 5’-TCG TCG TCR TTG CCC CAY TT-3’ |