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Table 2 Primers for anaerobic hydrocarbon degradation genes detection

From: Microbial diversity and anaerobic hydrocarbon degradation potential in an oil-contaminated mangrove sediment

Primer Set Forward (F) and Reverse (R) Oligonucleotide Primer Sequences Expected amplicon size (bp) Reference
SP9/ASP1 (bamA) F: 5`-CAG TAC AAY TCC TAC ACV ACB G-3` ~300 [20]
R: 5`-C MAT GCC GAT YTC CTG RC-3`
assA2F/R (assA) F: 5’-YAT GWA CTG GCA CGG MCA-3’ 440 Aitken et al., unpublished observations
R: 5’-GCR TTT TCM ACC CAK GTA-3’
7772 F/8546R (bssA) F: 5’-GAC ATG ACC GAC GCS ATY CT-3’ ~794 [22]
R: 5’-TCG TCG TCR TTG CCC CAY TT-3’
  1. Oligonucleotide primers used in PCR reactions for anaerobic hydrocarbon degradation detection.