Microbial diversity and anaerobic hydrocarbon degradation potential in an oil-contaminated mangrove sediment

BMC Microbiology

Table 1 Primers for sulphate-reducing bacteria detection

	Primer Set	Forward (F) and Reverse (R) Oligonucleotide Primer Sequences	Reference
Primer Set 1	DSR1F	F: 5’-ACS CAC TGG AAG CAC GGC GG-3’	[23]
Primer Set 1	DSR4R	R: 5’-GTG TAG CAG TTA CCG CA-3’	[36]
Primer Set 2	DSRp2060F-GC	F: 5’-CGC CCG CCG CGC CCC GCG CCC GGC CCG CCG CCC CCG CCC CCA ACA TCG TYC AYA CCC AGG G-3’	[36]
Primer Set 2	DSR4R	R: 5’-GTG TAG CAG TTA CCG CA-3’	[36]

Oligonucleotide primers used in PCR reactions for assessment of the sulphate-reducing bacterial communities and comparison between the 3 studied depths.

ISSN: 1471-2180