Table 1 Genus specific 16S rRNA targeted bacterial primers used in this study
From: Real-time analysis of gut flora in Entamoeba histolytica infected patients of Northern India
Sr no. | Genus | Primer sequence | PCR Product (bp) | Tm(ºC) | References |
|---|---|---|---|---|---|
1. | Methanobrevibactr | F 5’- CGATGCGGACTTGGTGTTG-3’ | 184 | 59.7 | [21] |
R 5’-TGTCGCCTCTGGTGAGATGTC-3’ |  | 59.8 |  | ||
2. | Peptostreptococcus | F 5’-AACTCCGGTGGTATCAGATG-3’ | 270 | 55.4 | [1] |
R 5’-GGGGCTTCTGAGTCAGGTA-3’ |  | 56.4 |  | ||
3. | Ruminococcus | F 5’-GAAAGCGTGGGGAGCAAACAGG-3’ | 302 | 65.8 | [21] |
R 5’- GACGACAACCATGCACCACCTG-3’ |  | 64.4 |  | ||
4. | Eubacterium | F 5’-GTAGTCCACGCCGTAAACGATG-3’ | 278 | 60.4 | [21] |
R 5’-ACACGAGCTGACGACAACCATG-3’ |  | 62.4 |  | ||
5. | Bacteroides | F 5’- GGGGTTCTGAGAGGAAG-3’ | 115 | 54.0 | [21] |
R 5’- GCTACTTGGCTGGTTCAG-3’ |  | 56.0 |  | ||
6. | Lactobacillus | F 5’-GCAGCAGTAGGGAATCTTCCA-3’ | 340 | 64.0 | [25] |
R 5’-GCATTYCACCGCTACACATG-3’ |  | 58.0 |  | ||
7. | Clostridium leptum subgroup | F 5’-CGTCAGCTCGTGTCGTGAGAT-3’ | 125 | 60.0 | [21] |
R 5’-CGTCATCCCCACCTTCCTCC-3’ |  | 62.5 |  | ||
8. | Clostridium coccoides subgroup | F 5’-GCCACATTGGGACTGAGA-3’ | 170 | 56.0 | This study |
R 5’-GCTTCTTAGTCAGGTACCG-3’ |  | 58.0 |  | ||
9. | Campylobacter | F 5’-AGGGAATATTGCGCAATGGGGGAAA-3’ | 180 | 58.0 | [21] |
R 5’- GATTCCGAGTAACGCTTGCACCCT-3’ |  | 59.0 |  | ||
10. | Bifidobacterium | F 5’-GATTCTGGCTCAGGATGAACGC-3’ | 231 | 61.9 | [21] |
R 5’-CTGATAGGACGCGACCCCAT-3’ |  | 60.8 |  | ||
11. | Sulfate-reducing bacteria (APS reductase subunit A gene) | F 5’-TGGCAGATMATGATYMACGG-3’ | 396 | 54. | This study |
R 5’-GGCCGTAACCGTCCTTGAA-3’ |  | 54.0 |  |