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Table 1 Genus specific 16S rRNA targeted bacterial primers used in this study

From: Real-time analysis of gut flora in Entamoeba histolytica infected patients of Northern India

Sr no. Genus Primer sequence PCR Product (bp) Tm(ºC) References
1. Methanobrevibactr F 5’- CGATGCGGACTTGGTGTTG-3’ 184 59.7 [21]
R 5’-TGTCGCCTCTGGTGAGATGTC-3’   59.8  
2. Peptostreptococcus F 5’-AACTCCGGTGGTATCAGATG-3’ 270 55.4 [1]
R 5’-GGGGCTTCTGAGTCAGGTA-3’   56.4  
3. Ruminococcus F 5’-GAAAGCGTGGGGAGCAAACAGG-3’ 302 65.8 [21]
R 5’- GACGACAACCATGCACCACCTG-3’   64.4  
4. Eubacterium F 5’-GTAGTCCACGCCGTAAACGATG-3’ 278 60.4 [21]
R 5’-ACACGAGCTGACGACAACCATG-3’   62.4  
5. Bacteroides F 5’- GGGGTTCTGAGAGGAAG-3’ 115 54.0 [21]
R 5’- GCTACTTGGCTGGTTCAG-3’   56.0  
6. Lactobacillus F 5’-GCAGCAGTAGGGAATCTTCCA-3’ 340 64.0 [25]
R 5’-GCATTYCACCGCTACACATG-3’   58.0  
7. Clostridium leptum subgroup F 5’-CGTCAGCTCGTGTCGTGAGAT-3’ 125 60.0 [21]
R 5’-CGTCATCCCCACCTTCCTCC-3’   62.5  
8. Clostridium coccoides subgroup F 5’-GCCACATTGGGACTGAGA-3’ 170 56.0 This study
R 5’-GCTTCTTAGTCAGGTACCG-3’   58.0  
9. Campylobacter F 5’-AGGGAATATTGCGCAATGGGGGAAA-3’ 180 58.0 [21]
R 5’- GATTCCGAGTAACGCTTGCACCCT-3’   59.0  
10. Bifidobacterium F 5’-GATTCTGGCTCAGGATGAACGC-3’ 231 61.9 [21]
R 5’-CTGATAGGACGCGACCCCAT-3’   60.8  
11. Sulfate-reducing bacteria (APS reductase subunit A gene) F 5’-TGGCAGATMATGATYMACGG-3’ 396 54. This study
R 5’-GGCCGTAACCGTCCTTGAA-3’   54.0