Figure 2
From: Real-time analysis of gut flora in Entamoeba histolytica infected patients of Northern India
Screening of Stool samples by PCR. (A) Schematic representation of location of Entamoeba histolytica specific primer. BH16197 is Genbank accession number of Entamoeba histolytica SINE-2 (EhSINE2) element; (B) Schematic representation of location of Entamoeba dispar specific primer from rDNA molecule. 18 S, 5.8 S and 28 S are corresponding ribosomal gene sequences and ITS-1 and ITS-2 refers to internal transcribed spacer 1 and 2; (C) Detection of E. histolytica in stool DNA sample using E. histolytica specific primers, Lane 1 = Marker 100 bp, Lane 2 = EhHM1 genomic DNA as positive control, Lane 3 & 4 stool sample DNA, Lane 5 = Genomic DNA of E. dispar SAW760 as negative control. Sample in lane 4 is E. histolytica positive; (D) Detection of E. dispar in Stool sample using E. dispar specific primers. Lane 1 = Marker 1 kb, Lane 2,3,4 and 5 stool sample DNA, Lane 6 = Genomic DNA of E. dispar as positive control. Sample in Lane 3 and 5 are E. dispar positive. Lane 4 stool sample is E. histolytica positive and was used as negative control.