Table 6 Strains and plasmids used in this study

Plasmids

pCM11

Plasmid stable in S. aureus that constitutively expresses green fluorescent protein (GFP); Em^r

Alexander Horswill, personal communication

pMRP9-1

pUCP-18 cloning vector carrying a GFP cassette; Cb^r

[56]

pMP7605

pBBR1MCS-5 cloning vector carrying the mCherry gene under the control of the tac promoter; Cb^r

[34]

Pseudomonas aeruginosa

PA103

Human isolate

[24]

PAK

Prototroph; human isolate

[57]

PAO1

Prototroph; human isolate

[58]

PAO-R1

ΔlasR derivative of PAO1; Tc^r

[51]

PAO-JP1

ΔlasI derivative of PAO1; Tc^r

[59]

PDO111

rhlR::Tn501 derivative of PAO1; Hg^r

[60]

PDO100

ΔrhlI::Tn501 derivative of PAO1; Hg^r

[60]

PW2798::pqsA-lacZ

pqsA-H05::ISlacZ/hah derivative of PAO1; Tc^r

[61]; University of Washington Genome Center

CI-4

Human isolate from chronic lower respiratory infection; ΔlasR, ΔrhlR

[27]

Staphylococcus aureus

AH133

RN4220 carrying pCM11; Em^r

[62]; Alexander Horswill, personal communication