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Table 6 Strains and plasmids used in this study

From: Characterization of biofilm-like structures formed by Pseudomonas aeruginosa in a synthetic mucus medium

Strain Description Source
Plasmids
pCM11 Plasmid stable in S. aureus that constitutively expresses green fluorescent protein (GFP); Emr Alexander Horswill, personal communication
pMRP9-1 pUCP-18 cloning vector carrying a GFP cassette; Cbr [56]
pMP7605 pBBR1MCS-5 cloning vector carrying the mCherry gene under the control of the tac promoter; Cbr [34]
Pseudomonas aeruginosa
PA103 Human isolate [24]
PAK Prototroph; human isolate [57]
PAO1 Prototroph; human isolate [58]
PAO-R1 ΔlasR derivative of PAO1; Tcr [51]
PAO-JP1 ΔlasI derivative of PAO1; Tcr [59]
PDO111 rhlR::Tn501 derivative of PAO1; Hgr [60]
PDO100 ΔrhlI::Tn501 derivative of PAO1; Hgr [60]
PW2798::pqsA-lacZ pqsA-H05::ISlacZ/hah derivative of PAO1; Tcr [61]; University of Washington Genome Center
CI-4 Human isolate from chronic lower respiratory infection; ΔlasR, ΔrhlR [27]
Staphylococcus aureus
AH133 RN4220 carrying pCM11; Emr [62]; Alexander Horswill, personal communication
  1. Em, erythromycin; r, resistant; Cb, carbenicillin; Hg, mercury; Tc, tetracycline.