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Table 3 Structural analysis of BLS formed by P. aeruginosa strains and QS mutants

From: Characterization of biofilm-like structures formed by Pseudomonas aeruginosa in a synthetic mucus medium

Strainsa Image stacks (#)b Total biovolume (μm3/μm2)b Mean thickness (μm)b Roughness coefficientd Total surface area × 107 (μm2)b Surface to volume ratio (μm2/μm3)b
Prototrophs and clinical isolate
PAO1 10 18.2 ± 0.69 17.5 ± 0.12 0.05 ± 0.01 0.73 ± 0.23 0.28 ± 0.07
PAK 10 13.7 ± 2.82 13.2 ± 2.62 0.05 ± 0.02 0.62 ± 0.05 0.27 ± 0.06
PA103 10 10.7 ± 0.08 12.6 ± 2.13 0.07 ± 0.03 1.32 ± 0.50 0.61 ± 0.21
CI-4 10 0.48 ± 0.17 0.77 ± 0.45 1.67 ± 0.12 0.23 ± 0.84 2.45 ± 0.02
Quorum-sensing mutants
PAO1 (wt) 10 18.2 ± 0.69 17.5 ± 0.12 0.05 ± 0.01 0.73 ± 0.23 0.21 ± 0.07
PAO-R1 (ΔlasR) 10 19.3 ± 0.43 18.0 ± 0.00 0.02 ± 0.00 0.43 ± 0.15 0.12 ± 0.04
PAO-JP1
lasI)
10 17.6 ± 1.45 17.8 ± 0.15 0.02 ± 0.02 0.65 ± 0.26 0.22 ± 0.11
PDO111 (ΔrhlR) 10 7.29 ± 0.10 8.26 ± 0.05 0.13 ± 0.01 1.10 ± 0.08 0.79 ± 0.04
PDO100 (ΔrhlI) 10 6.61 ± 2.25 8.65 ± 2.49 0.67 ± 0.12 0.98 ± 0.14 1.01 ± 0.23
PW2798cpqsA) 10 18.4 ± 0.30 17.7 ± 0.08 0.03 ± 0.01 0.70 ± 0.10 0.20 ± 0.03
  1. aAll strains carry pMRP9-1 and were grown for 3 d under 10% EO2 without shaking.
  2. bSee Table 1 for description of parameters.
  3. cPW2798::pqsA-lac.