Characterization of biofilm-like structures formed by Pseudomonas aeruginosa in a synthetic mucus medium

BMC Microbiology

Table 3 Structural analysis of BLS formed by P. aeruginosa strains and QS mutants

Strains^a	Image stacks (#)^b	Total biovolume (μm³/μm²)^b	Mean thickness (μm)^b	Roughness coefficient^d	Total surface area × 10⁷ (μm²)^b	Surface to volume ratio (μm²/μm³)^b
Prototrophs and clinical isolate
PAO1	10	18.2 ± 0.69	17.5 ± 0.12	0.05 ± 0.01	0.73 ± 0.23	0.28 ± 0.07
PAK	10	13.7 ± 2.82	13.2 ± 2.62	0.05 ± 0.02	0.62 ± 0.05	0.27 ± 0.06
PA103	10	10.7 ± 0.08	12.6 ± 2.13	0.07 ± 0.03	1.32 ± 0.50	0.61 ± 0.21
CI-4	10	0.48 ± 0.17	0.77 ± 0.45	1.67 ± 0.12	0.23 ± 0.84	2.45 ± 0.02
Quorum-sensing mutants
PAO1 (wt)	10	18.2 ± 0.69	17.5 ± 0.12	0.05 ± 0.01	0.73 ± 0.23	0.21 ± 0.07
PAO-R1 (ΔlasR)	10	19.3 ± 0.43	18.0 ± 0.00	0.02 ± 0.00	0.43 ± 0.15	0.12 ± 0.04
PAO-JP1 (ΔlasI)	10	17.6 ± 1.45	17.8 ± 0.15	0.02 ± 0.02	0.65 ± 0.26	0.22 ± 0.11
PDO111 (ΔrhlR)	10	7.29 ± 0.10	8.26 ± 0.05	0.13 ± 0.01	1.10 ± 0.08	0.79 ± 0.04
PDO100 (ΔrhlI)	10	6.61 ± 2.25	8.65 ± 2.49	0.67 ± 0.12	0.98 ± 0.14	1.01 ± 0.23
PW2798^c (ΔpqsA)	10	18.4 ± 0.30	17.7 ± 0.08	0.03 ± 0.01	0.70 ± 0.10	0.20 ± 0.03

^aAll strains carry pMRP9-1 and were grown for 3 d under 10% EO₂ without shaking.
^bSee Table 1 for description of parameters.
^cPW2798::pqsA-lac.

ISSN: 1471-2180