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Table 2 Significance of differences in values presented in Table 1

From: Characterization of biofilm-like structures formed by Pseudomonas aeruginosa in a synthetic mucus medium

Variablea

Image stacks (#)b

Total biovolume (μm3/μm2)b

Mean thickness (μm)b

Roughness coefficientb

Total surface area × 107 (μm2)b

Surface to volume ratio (μm2/μm3)b

Time (under 20 % EO 2 )

3d vs. 2d

10

Increasec 0.0002

Increase <0.0001

Decrease <0.0001

NSd

Decrease 0.0027

6d vs. 2d

10

Increase 0.0002

Increase 0.0002

Decrease <0.0001

Decrease <0.0001

Decrease <0.0001

Mucin concentration (3 d under 20 % EO 2 )

2.0X vs. 1X

10

Increase 0.0002

Increase 0.0003

Decrease 0.0006

NS

Decrease 0.0018

0.5X vs. 1X

10

Increase 0.0019

Increase 0.0007

Decrease 0.0011

Increase 0.0290

NS

DNA concentration (3 d under 20 % EO 2 )

1.5X vs. 1X

10

Decrease <0.0001

Decrease <0.0001

Increase <0.0001

Decrease <0.0001

Increase <0.0001

0.5X vs. 1X

10

Decrease <0.0001

Decrease 0.0002

Increase 0.0013

Decrease 0.0008

Increase 0.0124

Oxygen concentration (EO 2 ) e

10% vs. 20%

10

Increase <0.0001

Increase <0.0001

Decrease <0.0001

Decrease <0.0001

Decrease <0.0001

0% vs. 20%

10

Decrease <0.0001

Decrease <0.0001

Increase <0.0001

Decrease 0.0287

Increase 0.0482

  1. aAll strains carry pMRP9-1 and were grown without shaking.
  2. bSee Table 1 for description of parameters.
  3. cSignificant change with P value indicated below direction of change.
  4. dNS, no significant difference.
  5. e20%, aerobic; 10%, microaerobic; 0%, anaerobic; cultures were grown for 3 d, except 0% EO2 for 6d.