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Table 1 Effect of time and environmental variables on PAO1/pMRP9-1 BLS

From: Characterization of biofilm-like structures formed by Pseudomonas aeruginosa in a synthetic mucus medium

Variable Image stacks (#)a Total biovolume (μm3/μm2)b Mean thickness (μm)c Roughness coefficientd Total surface area × 107 (μm2)e Surface to volume ratio (μm2/μm3)f
Time (under 20% EO 2 )
48 h 10 6.52 ± 0.43 11.6 ± 0.28 0.53 ± 0.02 1.65 ± 0.24 1.54 ± 0.10
72 h 10 11.1 ± 0.40 15.5 ± 0.23 0.18 ± 0.02 2.15 ± 0.03 1.01 ± 0.04
6 d 10 18.2 ± 0.32 17.8 ± 0.06 0.02 ± 0.00 0.96 ± 0.12 0.28 ± 0.04
Mucin concentration (3 d under 20% EO 2 )
1X 10 11.1 ± 0.40 15.5 ± 0.23 0.18 ± 0.02 2.15 ± 0.03 1.01 ± 0.04
0.5X 10 13.5 ± 0.24 17.0 ± 0.05 0.08 ± 0.00 2.44 ± .045 0.94 ± 0.03
2X 10 15.4 ± 0.35 17.3 ± 0.08 0.06 ± 0.00 1.97 ± .098 0.67 ± 0.05
DNA concentration (3 d under 20% EO 2 )
1X 10 11.1 ± 0.40 15.5 ± 0.23 0.18 ± 0.02 2.15 ± 0.03 1.01 ± 0.04
0.5X 10 2.42 ± 0.54 4.37 ± 1.37 1.33 ± 0.20 0.76 ± .220 1.55 ± 0.15
1.5X 10 2.48 ± 0.22 5.52 ± 0.64 1.07 ± 0.07 0.96 ± .086 2.02 ± 0.01
Oxygen concentration (EO 2 ) g
20% 10 11.1 ± 0.40 15.5 ± 0.23 0.18 ± 0.02 2.15 ± 0.03 1.01 ± 0.04
10% 10 19.4 ± 0.28 17.9 ± 0.04 0.01 ± 0.00 0.46 ± 0.12 0.13 ± 0.03
0% 10 0.28 ± 0.19 0.41 ± 0.27 1.94 ± 0.04 0.07 ± 0.06 1.75 ± 0.30
  1. a Each experiment was done in duplicate. Two 10-image stacks were obtained from random positions within the BLs. A total of 40-image stacks were analyzed were analyzed using the COMSTAT program [20]. Values represent the mean ± SEM.
  2. bEstimates the biomass of the BLS.
  3. cMeasures spatial size of the BLS.
  4. dAssessment of the variation in the thickness of the BLS, or BLS heterogeneity.
  5. eTotal of the area occupied in each image stack.
  6. fEstimates the portion of the biofilm exposed to nutrients; biovolume divided by the surface area of the substratum.
  7. g20%, aerobic; 10%, microaerobic; 0%, anaerobic; cultures were grown for 3 d, except 0% EO2 for 6d.