Loss of individual QS genes affects BLS formation. PAO1 strains defective in the lasR (PAO-R1), lasI (PAO-JP1), rhlR (PDO111), rhlI (PDO100), or pqsA (PW2798::pqsA-lacZ) genes were transformed with pMRP9-1 and the transformants plus PAO1/pMRP9-1 as a control were grown in ASM+ under 10% EO2 without shaking for 3 d. The BLS were analyzed as described in Figure 3. (A and C) Representative micrographs of the BLS; magnification, 10X; bar, 200.00 nm. (B and D) Respective 3-D images constructed from the CLSM micrographs. Boxes, 800.00 px W x 600 px H; bars, 100 px.