Figure 6

The level of EO ₂ influences the development of PAO1 BLS in ASM+. Cells were inoculated into ASM+ and the cultures were incubated for 3 d under 20% or 10% EO₂. To obtain growth of PAO1 anaerobically, 10% potassium nitrate was added as a terminal electron acceptor and incubation continued for 6 d in 0% EO₂. The biofilms were analyzed as described in Figure 3. (A) CLSM micrographs of the BLS; magnification, 10X; bar, 200.00 nm. (B) The 3-D architecture of the BLS shown in (A); boxes, 800.00 px W x 600 px H; maximum depth, 20% EO₂ 88.00 μm, 10% EO₂ 217.00 μm, 0% EO₂ 56.00 μm; bar, 100 px.