PAO1 inhibits AH133 in co-culture. Overnight LB cultures of AH133 and PAO1/pMRP9-1 were pelleted, washed, and resuspended in PBS. Resuspended cells of each species were inoculated into ASM+ (A) or TSBDC (B) at an initial OD600 of approximately 0.015. The CFU/ml of each species was determined at the time of inoculation (0-time) and after 48 h of growth using selective media (Methods). The graphs show CFU/ml obtained from BLS in ASM+ (A) and planktonic growth in TSBDC (B). Values represent the means of at least three independent experiments ± SEM.