sigE facilitates the adaptation of Bordetella bronchiseptica to stress conditions and lethal infection in immunocompromised mice

Table 1 Strains and plasmids

	Strain name	Genotype	Source, Reference
E. coli	SEA001	MG1655 ΦλrpoHP3::lacZ ΔlacX74	[60]
	SEA5036	BL21(DE3) ΔslyD::kan pLysS pPER76	[61]
	XQZ001	BL21(DE3) ΔslyD::kan pLysS pXQZ001	This work
	SEA4114	CAG43113 ΔrpoE::kan ΔnadB::Tn10	[62]
	SEA008	SEA001 pTrc99a	[62]
	SEA5005	SEA001 pSEB006	This work
	XQZ003	DH5α pXQZ0003	This work
	SS1827	DH5α pSS1827	[63]
B. bronchiseptica	RB50	RB50	[58]
	SEA5516	RB50ΔsigE	This work
	MER001	RB50 pCW505	This work
	MER002	RB50ΔsigE pCW505	This work
	SEA5518	RB50 pEV	This work
	SEA5520	RB50ΔsigE pEV	This work
	SEA5526	RB50 pSigE	This work
	SEA5530	RB50ΔsigE pSigE	This work
	RB50Δwbm	RB50ΔwbmBwbmCwbmDwbmE	[64]
	WD3	RB50ΔbscN	[49]
	Plasmid name	Description	Source, Reference
	pTrc99a	Vector, pBR322 ori, Ap^R	Pharmacia
	pSEB006	sigE in pTrc99a	This work
	pSEB015	isolated rpoHP3 promoter in pRLG770, Ap^R	[61]
	pPER76	rpoE in T7 expression vector pET15b, Kan^R	[65]
	pXQZ001	sigE in T7 expression vector pET15b, Kan^R	This work
	pXQZ002	ΔsigE in TOPO-TA vector	This work
	pSS1827	helper plasmid competent for mating, Ap^R	[63]
	pSS3962	Bordetella-specific allelic exchange vector, Kan^R	Stibitz, unpublished work
	pXQZ003	ΔsigE in pSS3962	This work
	pEV	Vector pJS72, ΩSpec^R cassette replaced with Cm^R	This work
	pSigE	sigE in pEV	This work
	pCW505	cytoplasmic expression of GFP	[66]

ISSN: 1471-2180